Department of Ophthalmology, Kastamonu Eğitim Ve Araştırma Hastanesi, Kastamonu, Turkey.
Veni Vidi Eye Center, Istanbul, Turkey.
Biotech Histochem. 2022 Jul;97(5):334-339. doi: 10.1080/10520295.2021.1986133. Epub 2021 Oct 26.
Eyelid tumors commonly originate from the skin and its appendages. Environmental toxins and oxidants affect eyelid carcinogenesis. Glutathione S-transferases (GST) are antioxidants that participate in pathogenesis. We investigated GST levels in malignant and benign eyelid tumors in otherwise healthy individuals. We used 57 malignant eyelid biopsies, benign eyelid biopsies, and tissue removed during blepharoplasty and entropion operations culled from pathology archives. Specimens were divided into three groups: malignant lesions, benign lesions and controls consisting of eyelid tissue removed during routine blepharoplasty and entropion surgery. Specimens were immunostained for seven GST (GST-A, GST-P, GST-Z, GST-S, GST-K, GST-O, GST-T) and the intensity of staining was quantified. In the malignant group, GST-O and GST-P staining was less intense than for the control group. In the benign group, the GST-P level was less than for the control group. We found no significant difference between the intensity of staining in malignant and benign groups. Our findings suggest that GST-O and GST-P enzymes may play significant roles in eyelid carcinogenesis.
眼睑肿瘤通常起源于皮肤及其附属物。环境毒素和氧化剂会影响眼睑的癌变。谷胱甘肽 S-转移酶（GST）是一种抗氧化剂，参与发病机制。我们研究了在其他方面健康的个体中恶性和良性眼睑肿瘤中的 GST 水平。我们使用了 57 份恶性眼睑活检、良性眼睑活检以及从病理档案中挑选出的在眼睑成形术和倒睫手术中切除的组织。标本分为三组：恶性病变组、良性病变组和对照组，对照组由在常规眼睑成形术和倒睫手术中切除的眼睑组织组成。对标本进行了七种 GST（GST-A、GST-P、GST-Z、GST-S、GST-K、GST-O、GST-T）的免疫染色，并对染色强度进行了量化。在恶性组中，GST-O 和 GST-P 的染色强度低于对照组。在良性组中，GST-P 的水平低于对照组。我们没有发现恶性和良性组之间染色强度的显著差异。我们的研究结果表明，GST-O 和 GST-P 酶可能在眼睑癌变中发挥重要作用。
