Koyun Efe, Karadag Remzi, Ozkanli Seyma, Oguztuzun Serpil, Kocdogan Arzu Kaya, Ozsoy Isilay
Department of Ophthalmology, Kastamonu State Hospital, Kastamonu, Turkey.
Department of Ophthalmology, School of Medicine, Istanbul Medeniyet University, Goztepe, Istanbul, Turkey.
Postepy Dermatol Alergol. 2020 Aug;37(4):535-539. doi: 10.5114/ada.2020.98285. Epub 2020 Sep 2.
Eyelid tumours mostly originated from skin and its appendeges. External carcinogens like UV radiation causes cell damages in the eyelid skin and contributes to carcinogenesis. Apoptosis is a very important mechanism to prevent these damage and probable neoplatic change.
To compare caspase-3, p53 and Bcl-2 levels between patients with basal cell carcinoma (BCC) of the eyelid and healthy individuals.
Pathology archives from October 2012 to April 2015 were scanned for BCC biopsies of the eyelid and tissue removed during blepharoplasty and entropion procedures. A total of 36 specimens were found. The specimens were divided into two groups: BCC group and controls (consisting of eyelid tissue removed during routine blepharoplasty). The pathology specimens were then stained using p53, Bcl-2 and caspase-3 stains and the intensity of staining was graded on a 0-3 scale.
Samples from a total of 36 patients were included in the study. Eighteen (50.0%) patients were female. There were 13 patients in the BCC group and 23 patients in the control group. The mean age was 66.0 ±10.8 years in the BCC group, and 65.61 ±11.22 years in the control group. The caspase-3 staining was lower in the BCC group than in the control group. No significant differences were found between the BCC group and the control group in terms of p53 levels or Bcl-2 levels (both of them, = 1.000).
The caspase-3 level was lower in the BCC group. This result suggests that these enzymes can play a significant role in carcinogenesis of eyelid BCC.
眼睑肿瘤大多起源于皮肤及其附属器。紫外线辐射等外部致癌物会导致眼睑皮肤细胞损伤并促进癌变。细胞凋亡是预防这些损伤和可能的肿瘤性变化的非常重要的机制。
比较眼睑基底细胞癌（BCC）患者与健康个体之间的半胱天冬酶 - 3、p53和Bcl - 2水平。
扫描2012年10月至2015年4月的病理档案，查找眼睑BCC活检标本以及在眼睑成形术和睑内翻手术中切除的组织。共发现36个标本。标本分为两组：BCC组和对照组（由常规眼睑成形术中切除的眼睑组织组成）。然后使用p53、Bcl - 2和半胱天冬酶 - 3染色剂对病理标本进行染色，并将染色强度按0 - 3级进行分级。
本研究共纳入36例患者的样本。18例（50.０％）为女性。BCC组有13例患者，对照组有23例患者。BCC组的平均年龄为66.0±10.8岁，对照组为65.61±11.22岁。BCC组的半胱天冬酶 - 3染色低于对照组。BCC组和对照组在p53水平或Bcl - 2水平方面均未发现显著差异（两者P值均为1.000）。
BCC组的半胱天冬酶 - 3水平较低。这一结果表明这些酶在眼睑BCC的癌变过程中可能起重要作用。
