建立并鉴定转移性骨外尤文肉瘤小鼠模型。
Establishment and Characterisation of Metastatic Extraskeletal Ewing Sarcoma Mouse Models.
机构信息
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, Australia.
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, Australia
出版信息
In Vivo. 2021 Nov-Dec;35(6):3097-3106. doi: 10.21873/invivo.12604.
BACKGROUND/AIM: Ewing sarcomas most commonly arise in the bones, but can also manifest as extraskeletal tumours in soft tissues. Metastases from extraskeletal Ewing sarcomas occur in more diverse anatomical sites than skeletal tumours, and have poorer survival rates. Few animal models replicate the extraskeletal form of Ewing sarcoma, and those that have been developed do not reflect the widespread metastatic spread of these cancers.
MATERIALS AND METHODS
Luciferase-expressing Ewing sarcoma cells derived from a muscle tumour were intramuscularly or intravenously injected into nude mice.
RESULTS
Both models achieved metastatic spread to numerous sites including the lungs, liver, kidneys, and brain. We characterized the cellular composition of primary and metastatic tumours, observing a greater level of immune cell infiltration in metastases compared to primary intramuscular tumours.
CONCLUSION
These pre-clinical models will hopefully facilitate the evaluation of novel therapies and contribute to better understanding the disease progression of metastatic extraskeletal Ewing sarcoma.
背景/目的：尤文肉瘤最常发生于骨骼，但也可表现为软组织中的骨外肿瘤。骨外尤文肉瘤的转移发生在比骨骼肿瘤更多样的解剖部位，且生存率较低。很少有动物模型能复制尤文肉瘤的骨外形式，而那些已经开发的模型并不能反映这些癌症的广泛转移扩散。
材料和方法
从肌肉肿瘤中衍生出表达荧光素酶的尤文肉瘤细胞，通过肌肉内或静脉内注射到裸鼠体内。
结果
这两种模型都实现了向包括肺、肝、肾和脑在内的多个部位的转移。我们对原发和转移肿瘤的细胞组成进行了特征描述，观察到与原发肌肉内肿瘤相比，转移肿瘤中有更高水平的免疫细胞浸润。
结论
这些临床前模型有望促进新疗法的评估，并有助于更好地理解转移性骨外尤文肉瘤的疾病进展。
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