循环微颗粒在BCR/ABL1阴性骨髓增殖性肿瘤中与血栓形成相关的临床意义
The Clinical Significance of Circulating Microparticles Concerning Thrombosis in BCR/ABL1-negative Myeloproliferative Neoplasms.
作者信息
Aswad Mohamed Hussam, Kissova Jarmila, Ovesna Petra, Rihova Lucie, Penka Miroslav
机构信息
Department of Clinical Hematology, University Hospital Brno, Brno, Czech Republic;
Faculty of Medicine, Masaryk University, Brno, Czech Republic.
出版信息
In Vivo. 2021 Nov-Dec;35(6):3345-3353. doi: 10.21873/invivo.12632.
BACKGROUND/AIM: This work aimed to prospectively evaluate the clinical significance of circulating microparticles (MPs) in relation to thrombotic risk factors and thrombotic complications in patients with BCR/ABL1-negative myeloproliferative neoplasms (MPN).
PATIENTS AND METHODS
In a cohort of 206 patients with MPN, MPs' procoagulant activity was measured by the Zymuphen functional assay in 429 samples, while platelet- and erythrocyte-MPs were enumerated by flow cytometry in 558 samples.
RESULTS
MPN patients had higher MP levels than the control group. The levels of MPs were higher in male patients, smokers, and those who were older than 60 years, and in the presence of JAK2 mutation, history of thrombosis, platelets >400×10/l, hematocrit >45%, or leukocytes >10×10/l. Cytoreductive treatment reduced MP levels, with anagrelide being associated with lower MP levels than hydroxyurea.
CONCLUSION
The relationship with thrombotic risk factors indicates a possible role of MPs in the complex thrombotic mechanism, though cytoreductive treatment seems to affect this role through reducing MP levels.
背景/目的：本研究旨在前瞻性评估循环微颗粒（MPs）与BCR/ABL1阴性骨髓增殖性肿瘤（MPN）患者血栓形成危险因素及血栓形成并发症之间的临床意义。
患者与方法
在一组206例MPN患者中，采用Zymuphen功能分析法检测429份样本中MPs的促凝活性，同时采用流式细胞术对558份样本中的血小板微颗粒和红细胞微颗粒进行计数。
结果
MPN患者的MP水平高于对照组。男性患者、吸烟者、年龄大于60岁的患者以及存在JAK2突变、有血栓形成病史、血小板>400×10⁹/L、血细胞比容>45%或白细胞>10×10⁹/L的患者MP水平更高。细胞减灭治疗可降低MP水平，与羟基脲相比，阿那格雷与更低的MP水平相关。
结论
与血栓形成危险因素的关系表明MPs在复杂的血栓形成机制中可能发挥作用，尽管细胞减灭治疗似乎通过降低MP水平来影响这一作用。
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