Wallace J L, Whittle B J
Scand J Gastroenterol Suppl. 1986;125:79-85. doi: 10.3109/00365528609093821.
Topical application of hypertonic saline to a chambered gastric mucosa (ex vivo) resulted in the formation of a cap of mucus, fibrin, plasma, and cellular debris over the damaged regions. The role of this 'mucoid cap' in protecting the underlying mucosa from subsequent challenges with hypertonic saline was studied. In the control group, the repeated challenges with 1 M NaCl in 0.05 M HCl caused macroscopically visible gastric damage involving only 2% of the total glandular mucosa. In the test group, in which the mucoid cap was peeled off the mucosa after the first challenge with NaCl, there was significantly more mucosal leakage of albumin (p less than 0.05), and the macroscopically visible gastric damage progressively increased to involve over 14% of the total glandular mucosa by the end of the experiment (p less than 0.005). Histological analysis showed that the mucoid cap provided a protective layer, allowing for restitution of the epithelium and protection of the mucosa from the damaging effects of hypertonic saline. In the absence of this protective layer, the repeated exposure to hypertonic saline resulted in deep mucosal necrosis. These studies support the hypothesis that mucus released in response to acute damage plays an important role in protecting the underlying mucosa so that restitution of the epithelium can proceed.
将高渗盐水局部应用于有腔室的胃黏膜（离体），会在受损区域形成一层由黏液、纤维蛋白、血浆和细胞碎片组成的覆盖物。研究了这种“黏液帽”在保护下层黏膜免受高渗盐水后续刺激方面的作用。在对照组中，用0.05M盐酸中的1M氯化钠反复刺激，仅导致2%的总腺性黏膜出现肉眼可见的胃损伤。在试验组中，在用氯化钠首次刺激后将黏液帽从黏膜上剥离，白蛋白的黏膜渗漏显著增加（p小于0.05），到实验结束时，肉眼可见的胃损伤逐渐增加，累及超过14%的总腺性黏膜（p小于0.005）。组织学分析表明，黏液帽提供了一层保护层，使上皮得以修复，并保护黏膜免受高渗盐水的损伤作用。在没有这一保护层的情况下，反复暴露于高渗盐水会导致深层黏膜坏死。这些研究支持了这样一种假说，即急性损伤时释放的黏液在保护下层黏膜方面发挥着重要作用，从而使上皮得以修复。
