Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, China.
Mikrochim Acta. 2021 Oct 25;188(11):392. doi: 10.1007/s00604-021-05033-3.
Butyrylcholinesterase (BChE) is an important indicator for clinical diagnosis of liver dysfunction, organophosphate toxicity, and poststroke dementia. Point-of-care testing (POCT) of BChE activity is still a challenge, which is a critical requirement for the modern clinical diagnose. A portable photothermal BChE assay is proposed through modulating the photothermal effects of CuO nanoparticles. BChE can catalyze the decomposition of butyrylcholine, producing thiocholine, which further reduce and coordinate with CuO on surface of CuO nanoparticle. This leads to higher efficiency of formation of CuS nanoparticles, through the reaction between CuO nanoparticle and NaHS, together with the promotion of photothermal conversion efficiency from 3.1 to 59.0%, under the excitation of 1064 nm laser radiation. An excellent linear relationship between the temperature change and the logarithm of BChE concentration is obtained in the range 1.0 to 7.5 U/mL, with a limit of detection of 0.076 U/mL. In addition, the portable photothermal assay shows strong detection robustness, which endows the accurate detection of BChE in human serum, together with the screening and quantification of organophosphorus pesticides. Such a simple, sensitive, and robust assay shows great potential for the applications to clinical BChE detection and brings a new horizon for the development of temperature based POCT.
丁酰胆碱酯酶（BChE）是临床诊断肝功能障碍、有机磷毒性和中风后痴呆的重要指标。BChE 活性的即时检测（POCT）仍然是一个挑战，这是现代临床诊断的关键要求。通过调节氧化铜纳米粒子的光热效应，提出了一种便携式光热 BChE 分析方法。BChE 可以催化丁酰胆碱的分解，生成硫代胆碱，进一步还原并与氧化铜纳米粒子表面的 CuO 配位。这导致了 CuS 纳米粒子的形成效率更高，通过氧化铜纳米粒子与 NaHS 的反应，以及在 1064nm 激光辐射激发下光热转换效率从 3.1 提高到 59.0%。在 1.0 至 7.5 U/mL 的范围内，温度变化与 BChE 浓度的对数之间获得了优异的线性关系，检测限为 0.076 U/mL。此外，便携式光热分析具有很强的检测稳健性，能够准确检测人血清中的 BChE，同时筛选和定量有机磷农药。这种简单、灵敏、稳健的分析方法在临床 BChE 检测中的应用具有巨大的潜力，为基于温度的 POCT 的发展带来了新的前景。
