Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China.
Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Mar 15;269:120756. doi: 10.1016/j.saa.2021.120756. Epub 2021 Dec 14.
Butyrylcholinesterase (BChE) is an important clinical diagnosing index for liver dysfunction and organophosphate toxicity. However, the current assays for BChE activity are suffering from the relative poor detection sensitivity. In this work, an ultrasensitive fluorescence assay for BChE activity was developed based on the self-polymerization modulated fluorescence of sulfur quantum dots (S-dots). The luminescence of S-dots can be quenched by the self-polymerized dopamine. The hydrolysate of substrates, thiocholine, under the catalysis of BChE can reduce dopamine, which results in the inhibition of self-polymerization and the fluorescence recovery of S-dots. BChE can be quantitatively detected by recording the recovered fluorescence of S-dots, and a linear relationship is observed between the ratio of fluorescence and the concentration of BChE in the range from 0.01 to 10 U/L. A limit of detection as low as 0.0069 U/L calculated, which is the lowest number so far. The assay also shows excellent selectivity towards various interference species and acetylcholinesterase. These features allowed the direct detection of BChE activity in human serum, demonstrating the great practical applications of our assay.
丁酰胆碱酯酶（BChE）是肝功能障碍和有机磷毒性的重要临床诊断指标。然而，目前用于检测 BChE 活性的方法存在相对较差的检测灵敏度。在这项工作中，我们基于硫量子点（S-dots）的自聚合调控荧光，开发了一种用于检测 BChE 活性的超灵敏荧光分析法。S-dots 的荧光可以被自聚合的多巴胺猝灭。在 BChE 的催化作用下，底物的水解产物硫代胆碱可以还原多巴胺，从而抑制自聚合并恢复 S-dots 的荧光。通过记录 S-dots 的恢复荧光，可以定量检测 BChE，并且在 0.01 到 10 U/L 的范围内，荧光与 BChE 浓度之间呈线性关系。计算得到的检测限低至 0.0069 U/L，这是迄今为止的最低值。该测定法对各种干扰物质和乙酰胆碱酯酶也表现出优异的选择性。这些特点使得能够直接检测人血清中的 BChE 活性，展示了我们测定法的巨大实际应用价值。
