整合素靶向荧光剂 IntegriSense750 的高亮度准确预测皮瓣坏死，优于吲哚菁绿。
Hyperintensity of integrin-targeted fluorescence agent IntegriSense750 accurately predicts flap necrosis compared to Indocyanine green.
机构信息
Department of Otolaryngology - Head & Neck Surgery, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Liverpool Head and Neck Centre, Aintree University Hospital, University of Liverpool, Liverpool, UK.
出版信息
Head Neck. 2022 Jan;44(1):134-142. doi: 10.1002/hed.26914. Epub 2021 Oct 25.
BACKGROUND
Flap necrosis is a feared complication of reconstructive surgery. Current methods of prediction using Indocyanine green (ICG) lack specificity. IntegriSense750 is a fluorescence agent that binds sites of vascular remodeling. We hypothesized that IntegriSense750 better predicts flap compromise compared to ICG.
METHODS
Fifteen mice underwent lateral thoracic artery axial flap harvest. Mice received an injection of ICG (n = 7) or IntegriSense750 (n = 8) daily from postoperative days (POD) 0-3 and were imaged daily. Mean signal-to-background ratios quantified the change in fluorescence as necrosis progressed.
RESULTS
Mean signal-to-background ratio was significantly higher for IntegriSense750 compared to ICG on POD0 (1.47 ± 0.17 vs. 0.86 ± 0.21, p = 0.01) and daily through POD3 (2.12 ± 0.70 vs. 0.96 ± 0.29, p < 0.001).
CONCLUSIONS
IntegriSense750 demonstrates increased signal-to-background ratio at areas of flap distress compared to ICG which may increase identification of flap necrosis and improve patient outcomes.
背景
皮瓣坏死是重建手术中一种令人担忧的并发症。目前使用吲哚菁绿（ICG）进行预测的方法特异性不足。IntegriSense750 是一种荧光剂，可结合血管重塑部位。我们假设 IntegriSense750 比 ICG 更能预测皮瓣失代偿。
方法
15 只小鼠接受了胸外侧动脉轴形皮瓣采集。小鼠在术后第 0-3 天每天接受 ICG（n=7）或 IntegriSense750（n=8）注射，并每天进行成像。平均信号与背景比量化了荧光随坏死进展的变化。
结果
在第 0 天（1.47±0.17 与 0.86±0.21，p=0.01）和第 3 天（2.12±0.70 与 0.96±0.29，p<0.001），IntegriSense750 的平均信号与背景比明显高于 ICG。
结论
与 ICG 相比，IntegriSense750 在皮瓣受损区域显示出更高的信号与背景比，这可能增加对皮瓣坏死的识别并改善患者预后。
Zotero 插件