Zhou Shanyue, Yang Qianqian, Yin Changfa, Liu Lin, Liang Wenxing
College of Agronomy and Plant Protection, The Key Lab of Integrated Crop Pests Management of Shandong Province, Qingdao Agricultural University, No. 700 Changcheng Road, Chengyang, Qingdao, Shandong, 266109, China.
College of Plant Protection, China Agricultural University, Beijing, 100193, China.
BMC Genomics. 2016 Dec 13;17(1):1019. doi: 10.1186/s12864-016-3361-3.
Lysine acetylation in proteins is a ubiquitous and conserved post-translational modification, playing a critical regulatory role in almost every aspect of living cells. Although known for many years, its function remains elusive in Fusarium graminearum, one of the most important necrotrophic plant pathogens with huge economic impact.
By the combination of affinity enrichment and high-resolution LC-MS/MS analysis, large-scale lysine acetylome analysis was performed. In total, 577 lysine acetylation sites matched to 364 different proteins were identified. Bioinformatics analysis of the acetylome showed that the acetylated proteins are involved in a wide range of cellular functions and exhibit diverse subcellular localizations. Remarkably, 10 proteins involved in the virulence or DON (deoxynivalenol) biosynthesis were found to be acetylated, including 4 transcription factors, 4 protein kinases and 2 phosphatases. Protein-protein interaction network analysis revealed that acetylated protein complexes are involved in diversified interactions.
This work provides the first comprehensive survey of a possible lysine acetylome in F. graminearum and reveals previously unappreciated roles of lysine acetylation in the regulation of diverse biological processes. This work provides a resource for functional analysis of acetylated proteins in filamentous fungi.
蛋白质中的赖氨酸乙酰化是一种普遍存在且保守的翻译后修饰，在活细胞的几乎每个方面都发挥着关键的调节作用。尽管已被知晓多年，但其在禾谷镰刀菌（一种对经济有巨大影响的重要坏死营养型植物病原体）中的功能仍不清楚。
通过亲和富集与高分辨率液相色谱 - 串联质谱分析相结合，进行了大规模赖氨酸乙酰化蛋白质组分析。总共鉴定出577个赖氨酸乙酰化位点，它们与364种不同的蛋白质相匹配。对乙酰化蛋白质组的生物信息学分析表明，乙酰化蛋白质参与广泛的细胞功能，并表现出多样的亚细胞定位。值得注意的是，发现10种参与毒力或脱氧雪腐镰刀菌烯醇（DON）生物合成的蛋白质被乙酰化，包括4种转录因子、4种蛋白激酶和2种磷酸酶。蛋白质 - 蛋白质相互作用网络分析表明，乙酰化蛋白质复合物参与多种相互作用。
这项工作首次全面调查了禾谷镰刀菌中可能存在的赖氨酸乙酰化蛋白质组，并揭示了赖氨酸乙酰化在调节多种生物学过程中以前未被认识到的作用。这项工作为丝状真菌中乙酰化蛋白质的功能分析提供了资源。
