Fisheries College, Zhejiang Ocean University, Zhoushan 316022, China.
Curr Issues Mol Biol. 2021 Sep 25;43(3):1282-1292. doi: 10.3390/cimb43030091.
forms one of the most important commercial fisheries along the Bay of Bengal and the southeast coast of China. In this study, the genome-wide survey dataset first produced using next-generation sequencing (NGS) was used to provide general information on the genome size, heterozygosity and repeat sequence ratio of . About 68.74 GB of high-quality sequence data were obtained in total and the genome size was estimated to be 1315 Mb with the 17-mer frequency distribution. The sequence repeat ratio and heterozygosity were calculated to be 52.49% and 0.67%, respectively. A total of 1,027,651 microsatellite motifs were identified and dinucleotide repeat was the most dominant simple sequence repeat (SSR) motif with a frequency of 54.35%. As a by-product of whole genome sequencing, the mitochondrial genome is a powerful tool to investigate the evolutionary relationships between and its relatives. The maximum likelihood (ML) phylogenetic tree was constructed according to the concatenated matrix of amino acids translated from the 13 protein-coding genes (PCGs). Monophyly of two species of the genus was revealed in the present study and they formed a monophyletic clade with with a high bootstrap value of 100%. The results would help to push back the frontiers of genomics and open the doors of molecular diversity as well as conservation genetics studies on this species.
是孟加拉湾和中国东南沿海最重要的商业渔业之一。在这项研究中，首次使用下一代测序（NGS）生成的全基因组调查数据集，提供了关于基因组大小、杂合率和重复序列比例的一般信息。总共获得了约 68.74GB 的高质量序列数据，估计基因组大小为 1315Mb，17-mer 频率分布。序列重复率和杂合率分别计算为 52.49%和 0.67%。总共鉴定出 1027651 个微卫星基序，二核苷酸重复是最主要的简单重复序列（SSR）基序，频率为 54.35%。作为全基因组测序的副产品，线粒体基因组是研究与及其亲缘关系之间进化关系的有力工具。根据从 13 个蛋白质编码基因（PCGs）翻译的氨基酸串联矩阵构建了最大似然（ML）系统发育树。本研究揭示了两个属的单系性，它们与形成一个单系分支，支持率为 100%。研究结果将有助于推动基因组学的前沿发展，为该物种的分子多样性和保护遗传学研究打开大门。
