Xu T J, Sun D Q, Li H Y, Wang R X
Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan, PR China.
Genet Mol Res. 2011;10(3):1701-6. doi: 10.4238/vol10-3gmr1045.
The Bombay duck, or bummalo (Harpadon nehereus), is a lizardfish native to the Arabian sea, but also common in the China sea. It is normally dried and salted before consumption and export. To provide molecular information on this economically important fish species, we developed and characterized microsatellite markers. Ninety positive clones from the (CA)(15)-enriched genomic library were sequenced; 62 sequences contained sufficient repeat motifs (di-, tri- and tetra-nucleotide). Twenty-eight primer pairs were designed and 21 were successfully amplified; five loci were polymorphic, but with a low number of alleles (three or four). The observed and expected heterozygosities ranged from 0.3500 to 0.8421 and from 0.5244 to 0.6244, respectively. All of the five polymorphic loci were at Hardy-Weinberg equilibrium (adjusted), while linkage disequilibrium between Hane-97 and Hane-175 (P< 0.05, adjusted P value = 0.01) was significant. The low degree of polymorphism of microsatellite markers may be due to the large size of the repeat motifs. These markers will be useful for genetic diversity analysis of H. nehereus.
龙头鱼，又称九肚鱼（Harpadon nehereus），是一种原产于阿拉伯海的狗母鱼，但在中国海也很常见。它通常在食用和出口前进行干燥和腌制。为了提供关于这种具有重要经济价值的鱼类的分子信息，我们开发并鉴定了微卫星标记。对来自（CA）(15)富集基因组文库的90个阳性克隆进行了测序；62个序列包含足够的重复基序（二核苷酸、三核苷酸和四核苷酸）。设计了28对引物，其中21对成功扩增；5个位点具有多态性，但等位基因数量较少（三个或四个）。观察到的杂合度和预期杂合度分别为0.3500至0.8421和0.5244至0.6244。所有5个多态性位点均处于哈迪-温伯格平衡（校正后），而Hane-97和Hane-175之间的连锁不平衡显著（P < 0.05，校正P值 = 0.01）。微卫星标记的多态性程度较低可能是由于重复基序的长度较大。这些标记将有助于九肚鱼的遗传多样性分析。
