Tada I, Korenaga M, Shiwaku K, Ogunba E O, Ufomadu G O, Nwoke B E
Am J Trop Med Hyg. 1987 Mar;36(2):383-6. doi: 10.4269/ajtmh.1987.36.383.
The cross-reactivity of the blood from onchocerciasis, loiasis, and dipetalonemiasis was tested by a micro-ELISA technique, utilizing adult Onchocerca volvulus antigen and blood samples taken on filter paper. The average ELISA values (OD at 500 nm) were as follows: 0.58 in persons with O. volvulus microfilariae (n = 81), 0.49 in microfilariae-negatives from the same endemic area (n = 39), 0.15 in dipetalonemiasis (n = 27), and 0.25 in loiasis (n = 12), while those of 65 Dipetalonema perstans-negative people were markedly low (average 0.14) and that of 22 Loa loa-negatives, 0.22, respectively. This ELISA could successfully differentiate onchocerciasis from dipetalonemiasis and loiasis.
利用旋盘尾丝虫成虫抗原和滤纸采集的血样，通过微量酶联免疫吸附测定（ELISA）技术检测了盘尾丝虫病、罗阿丝虫病和双瓣丝虫病患者血液的交叉反应性。ELISA的平均数值（500nm处的光密度）如下：旋盘尾丝虫微丝蚴感染者为0.58（n = 81），同一流行地区无微丝蚴者为0.49（n = 39），双瓣丝虫病患者为0.15（n = 27），罗阿丝虫病患者为0.25（n = 12），而65例阴性双瓣丝虫病患者的数值明显较低（平均0.14），22例阴性罗阿丝虫病患者的数值为0.22。该ELISA能够成功区分盘尾丝虫病与双瓣丝虫病和罗阿丝虫病。
