Weich H A, Herbst D, Schairer H U, Hoppe J
FEBS Lett. 1987 Mar 9;213(1):89-94. doi: 10.1016/0014-5793(87)81470-9.
It was shown previously [(1984) EMBO J. 3, 453-459] that after treatment of the human erythroleukemia cell line HEL with phorbol ester and dimethyl sulfoxide there was a marked increase in the amounts of megakaryocytic markers, especially of platelet alpha-granule proteins and platelet glycoproteins. In order to investigate this differentiation process further we have studied the expression of the mRNA encoding PDGF-A and PDGF-B (c-sis). Upon addition of the phorbol ester to the culture medium the expression of the c-sis transcript was enhanced about 7-fold over a period of 4 days. With dimethyl sulfoxide there was no significant stimulation of the expression. Addition of cycloheximide to HEL cells treated for a short period with phorbol ester superinduced the expression of the c-sis gene. The HEL cells did not express the A-chain mRNA even in the presence of phorbol ester or dimethyl sulfoxide. This leads us to propose that synthesis of the PDGF-A chain and PDGF-B chain is differentially regulated in the megakaryocytic-like HEL cell line.
先前研究表明[（1984年）《欧洲分子生物学组织杂志》3, 453 - 459页]，用人红白血病细胞系HEL经佛波酯和二甲基亚砜处理后，巨核细胞标志物的量显著增加，尤其是血小板α-颗粒蛋白和血小板糖蛋白。为了进一步研究这一分化过程，我们研究了编码血小板衍生生长因子A（PDGF - A）和血小板衍生生长因子B（PDGF - B，即c - sis）的mRNA的表达。向培养基中添加佛波酯后，c - sis转录本的表达在4天内增强了约7倍。使用二甲基亚砜时，表达没有受到显著刺激。向短期经佛波酯处理的HEL细胞中添加环己酰亚胺可超诱导c - sis基因的表达。即使在存在佛波酯或二甲基亚砜的情况下，HEL细胞也不表达A链mRNA。这使我们提出，在类巨核细胞的HEL细胞系中，PDGF - A链和PDGF - B链的合成受到不同的调控。
