Papayannopoulou T, Raines E, Collins S, Nakamoto B, Tweeddale M, Ross R
J Clin Invest. 1987 Mar;79(3):859-66. doi: 10.1172/JCI112895.
We have examined the constitutive and inducible secretion of platelet-derived growth factor (PDGF)-like proteins in a variety of human hemopoietic cell lines. The highest levels of secreted protein were noted in four human erythroleukemia lines which, in addition to erythroid lineage markers, express one or more megakaryocytic lineage markers. Induction of these lines by 12-O-tetradecanoylphorbol-13-acetate enhanced the expression of megakaryocytic markers and increased secretion of PDGF-like proteins several fold. In concert with these changes, there was significant induction of c-sis/PDGF-B messenger RNA (mRNA) expression in all lines, whereas one line showed significant concurrent induction of PDGF-A mRNA expression. Whether PDGF-like secretion is part of the stem cell-like phenotype displayed by these lines or is secondary to their leukemic transformation remains to be determined. Nevertheless, these lines provide new cellular models for studying the expression and function of PDGF analogs in hemopoietic cells.
我们已经检测了多种人类造血细胞系中血小板衍生生长因子（PDGF）样蛋白的组成性分泌和诱导性分泌。在四种人类红白血病细胞系中观察到最高水平的分泌蛋白，这些细胞系除了表达红系谱系标志物外，还表达一种或多种巨核细胞系标志物。用12 - O - 十四酰佛波醇 - 13 - 乙酸酯诱导这些细胞系可增强巨核细胞标志物的表达，并使PDGF样蛋白的分泌增加数倍。与这些变化一致，所有细胞系中c - sis/PDGF - B信使核糖核酸（mRNA）表达均有显著诱导，而其中一个细胞系同时显示出PDGF - A mRNA表达的显著诱导。PDGF样分泌是这些细胞系所表现出的干细胞样表型的一部分，还是白血病转化的继发结果，仍有待确定。然而，这些细胞系为研究PDGF类似物在造血细胞中的表达和功能提供了新的细胞模型。
