口腔发育异常中Stathmin的免疫组织化学表达：一项关于其对微管作用的原创性研究。

Immunohistochemical expression of stathmin in oral dysplasia: An original study with an insight of its action on microtubules.

作者信息

Vadla Purnima, Deepthi G, Kumar Chippalapally Arun, Bashamalla Rithika, Syeda Neelam, Naramala Srikanth

机构信息

Department of Oral Pathology and Microbiology, Mamata Dental College, Khammam, Telangana, India.

出版信息

J Oral Maxillofac Pathol. 2021 May-Aug;25(2):247-252. doi: 10.4103/0973-029X.325122. Epub 2021 Aug 31.

DOI:10.4103/0973-029X.325122

PMID:34703117

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8491364/

Abstract

BACKGROUND AND OBJECTIVES

Stathmin is a phosphoprotein, which in its phosphorylated/unphosphorylated states plays a major role in polymerization/depolymerization of microtubules, respectively. Assembly of microtubules is an important aspect of cell division called mitosis. Hinderance in the function of stathmin could lead to damage in the mitotic process resulting in aneuploidy which is common manifestation of malignancies. Hence, stathmin could be used as a tumor marker for oral dysplasias and cancers. The purpose of the study is to compare the expression of stathmin in normal subjects to the patients with oral leukoplakia and to correlate its expression with different histopathological grades of oral leukoplakia This is the first ever study conducted to examine the expression of stathmin in oral dysplasia.

METHODOLOGY

Thirty histopathologically confirmed cases of oral dysplasia were selected for the study. These tissues were evaluated immunohistochemically for stathmin. To enumerate the stathmin stained cells, 300 cells were examined manually in at least 5 areas and a mean percentage of positive-stained slides were determined. Then, each sample was assigned to one of the following staining scores: (0) - (<10% of stained cells); (1) - (11%-25% of stained cells); (2) - (26%-50% of stained cells); (3) - (51%-75% of stained cells); (4) - (76%-90% of stained cells) and (5) - (91%-100% of stained cells). The results were analyzed statistically using ANNOVA test.

RESULTS

When comparison was made with respect to staining scores of stathmin between normal and dysplasia groups, the results were found to be statistically significant with a = 0.0001. A statistically significant difference was observed between various histopathological grades of dysplasia with respect to stathmin immunohistochemistry scores with a = 0.0001.

CONCLUSION

These results suggest stathmin as a tumor marker and prognostic indicator.

摘要

背景与目的

Stathmin是一种磷蛋白，其磷酸化/未磷酸化状态分别在微管的聚合/解聚中起主要作用。微管组装是细胞分裂（即有丝分裂）的一个重要方面。Stathmin功能的阻碍可能导致有丝分裂过程受损，从而导致非整倍体，这是恶性肿瘤的常见表现。因此，Stathmin可作为口腔发育异常和癌症的肿瘤标志物。本研究的目的是比较正常受试者与口腔白斑患者中Stathmin的表达情况，并将其表达与口腔白斑的不同组织病理学分级相关联。这是首次进行的研究，旨在检测Stathmin在口腔发育异常中的表达。

方法

选择30例经组织病理学确诊的口腔发育异常病例进行研究。对这些组织进行Stathmin的免疫组织化学评估。为了计数Stathmin染色的细胞，在至少5个区域手动检查300个细胞，并确定阳性染色切片的平均百分比。然后，将每个样本指定为以下染色分数之一：（0）-（<10%的染色细胞）；（1）-（11%-25%的染色细胞）；（2）-（26%-50%的染色细胞）；（3）-（51%-75%的染色细胞）；（4）-（76%-90%的染色细胞）和（5）-（91%-100%的染色细胞）。使用方差分析检验对结果进行统计学分析。