Smith H S, Wolman S R, Dairkee S H, Hancock M C, Lippman M, Leff A, Hackett A J
J Natl Cancer Inst. 1987 Apr;78(4):611-5.
The properties in culture of 3 breast cancer effusion metastases, obtained over approximately 2 years from the same patient, were examined. Despite repeated attempts with cryopreserved cells, only the last specimen reproducibly exhibited immortality in culture; the first 2 specimens grew initially but failed to develop into cell lines. Each specimen was unique in morphology and growth properties, although karyotypic markers indicated a common origin. Aberrations of chromosomes 1 and 11 marked these near-diploid cells, and further structural alterations of chromosome 11 accompanied the transition of biological properties observed in the third specimen.
对从同一患者身上在大约两年时间内获取的3个乳腺癌积液转移灶的培养特性进行了研究。尽管对冻存细胞进行了多次尝试，但只有最后一个标本在培养中可重复性地表现出永生化；前两个标本最初生长，但未能发育成细胞系。每个标本在形态和生长特性上都是独特的，尽管核型标记表明它们有共同的起源。1号和11号染色体的畸变标记了这些近二倍体细胞，并且在第三个标本中观察到的生物学特性转变伴随着11号染色体的进一步结构改变。
