用于癌基因转染研究的人类受体细胞。

Tainsky M A, Shamanski F L, Blair D, Vande Woude G

Mol Cell Biol. 1987 Mar;7(3):1280-4. doi: 10.1128/mcb.7.3.1280-1284.1987.

We used human oncogene DNA to transform the nontumorigenic, revertant, human osteosarcoma cell line HOS TE-85 clone 5 (ATCC CRL 1543) to tumorigenicity in athymic nude mice with latency periods as short as 3 weeks. These cells were also transformed by genetic markers in genomic DNA samples. Because of their low rate of spontaneous tumor formation and the simplicity of culturing them, HOS cells provide a human cell alternative to NIH 3T3 murine fibroblasts for oncogene transfection studies.

我们使用人类致癌基因DNA，将无致瘤性的、回复突变的人类骨肉瘤细胞系HOS TE - 85克隆5（ATCC CRL 1543）在无胸腺裸鼠中转化为具有致瘤性，潜伏期短至3周。这些细胞也被基因组DNA样本中的遗传标记所转化。由于其自发肿瘤形成率低且培养简单，HOS细胞为致癌基因转染研究提供了一种替代NIH 3T3小鼠成纤维细胞的人类细胞。