人神经母细胞瘤细胞系转化基因的分离与初步鉴定

Shimizu K, Goldfarb M, Perucho M, Wigler M

Proc Natl Acad Sci U S A. 1983 Jan;80(2):383-7. doi: 10.1073/pnas.80.2.383.

DNA from the human neuroblastoma cell line SK-N-SH is capable of inducing foci of transformed NIH 3T3 cells after DNA-mediated gene transfer. Using genetic selection with the Escherichia coli sup F gene, we have isolated human sequences from mouse cells responsible for the oncogenic transformation. These sequences are present in all human DNAs surveyed and no gross rearrangements of these sequences are found in SK-N-SH cells. Although clearly distinct from two other human transforming genes present in bladder, lung, and colon carcinoma cell lines, all three transforming gene sequences may be related members of the ras gene family.

人类神经母细胞瘤细胞系SK-N-SH的DNA在DNA介导的基因转移后能够诱导NIH 3T3转化细胞形成集落。利用大肠杆菌supF基因进行遗传筛选，我们从小鼠细胞中分离出了负责致癌转化的人类序列。这些序列存在于所有检测的人类DNA中，并且在SK-N-SH细胞中未发现这些序列的明显重排。尽管与存在于膀胱、肺和结肠癌细胞系中的另外两个人类转化基因明显不同，但这三个转化基因序列可能是ras基因家族的相关成员。