School of Materials Science and Engineering, School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
China Nonferrous Metal (Guilin) Geology for Mineral Co., Ltd., Guilin, Guangxi 541004, China.
J Biomed Nanotechnol. 2021 Oct 1;17(10):2043-2052. doi: 10.1166/jbn.2021.3177.
A persimmon tannin- composite powder (PT-A) was investigated for its capacity to protect against ionizing radiation. Human hepatic cells (L02 cells) and human hepatoma cells (HepG2 cells) were pretreated with different concentrations of PT-A or the single compounds (PT or ) and radiated with X-rays. After radiation and post-incubation for 12 h or 24 h, the cell viability, apoptosis, and reactive oxygen species (ROS) production were analyzed by Cell Counting Kit 8 (CCK-8), 2',7'-dichlorfluorescein diacetate (DCFH-DA) staining, and Hoechst 33258 staining/flow cytometry, respectively. CCK-8 results illustrated that the optimal radiation dose L02 cells was 8 Gy for L02 cells, and the cell activity was 71.72% (IC = 412.1 μg/mL) after post-radiation incubation of 12 h. For HepG2 cells, the optimal radiation dose was 8 Gy, and the cell activity was 62.37% (IC = 213.0 μg/mL). The cell apoptotic rate was the lowest at a PT-A concentration of 200 μg/mL in L02 cells (4.32%, < 0.05), and at 100 μg/mL in HepG2 cells (9.80%, < 0.05). ROS production induced by radiation could be effectively inhibited by 200 μg/mL of PT-A in L02 cells, and by 100 μg/mL of PT-A in HepG2 cells. The PT-A composite has good radioprotective effects on cell vitality and apoptosis of X-rays radiation exposure towards L02 cells and HepG2 cells compared to the persimmon tannin or . Therefore, PT-A composite might be useful as a natural, harmless anti-ionizing radiation agent, and has various clinical application prospects in future.
柿单宁复合粉（PT-A）的抗电离辐射能力研究。用不同浓度的 PT-A 或单一化合物（PT 或 ）预处理人肝细胞（L02 细胞）和人肝癌细胞（HepG2 细胞），并用 X 射线照射。照射后孵育 12 h 或 24 h，用细胞计数试剂盒 8（CCK-8）、2'，7'-二氯荧光素二乙酸酯（DCFH-DA）染色和 Hoechst 33258 染色/流式细胞术分别分析细胞活力、细胞凋亡和活性氧（ROS）的产生。CCK-8 结果表明，L02 细胞的最佳辐射剂量为 8 Gy，辐射后 12 h 孵育，细胞活力为 71.72%（IC = 412.1 μg/mL）。对于 HepG2 细胞，最佳辐射剂量为 8 Gy，细胞活力为 62.37%（IC = 213.0 μg/mL）。在 L02 细胞中，PT-A 浓度为 200 μg/mL 时细胞凋亡率最低（4.32%，<0.05），在 HepG2 细胞中，PT-A 浓度为 100 μg/mL 时细胞凋亡率最低（9.80%，<0.05）。200 μg/mL 的 PT-A 可有效抑制 L02 细胞中辐射诱导的 ROS 产生，100 μg/mL 的 PT-A 可有效抑制 HepG2 细胞中辐射诱导的 ROS 产生。与柿单宁或 相比，PT-A 复合剂对 L02 细胞和 HepG2 细胞的 X 射线照射后细胞活力和细胞凋亡具有较好的放射保护作用。因此，PT-A 复合剂可能作为一种天然、无害的抗电离辐射剂具有广阔的临床应用前景。
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