School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin, China.
China Nonferrous Metal (Guilin) Geology for Mineral Co., Ltd, Guilin, China.
Pharm Biol. 2020 Dec;58(1):510-517. doi: 10.1080/13880209.2020.1767158.
Persimmon tannin (extract of L.f [Ebenaceae]) and gel extract of (L.) Burm.f. [Asphodelaceae]) are known as anti-radiation agents. However, radiation resistance of the persimmon tannin- gel composite remains inconclusive. To investigate the capacity of the persimmon tannin- gel composite to protect against ionising radiation at the cellular level. HaCaT (human epidermal keratinocytes) cells were pre-treated with PT-A-1 (the mass ratio of persimmon tannin and gel was 2:1) or the single component (persimmon tannin or gel) at various concentrations (0, 50, 100, 200, 400, 800 μg/mL. Control group: medium with no HaCaT cells), and then radiated with X-rays (radiation dose: 4, 8, 12, 16, and 20 Gy). Cell viability, cell apoptosis, and radiation-induced intracellular reactive oxygen species (ROS) generation were analysed by CCK-8, Hoechst 33258 staining/flow cytometry, and 2',7'-dichlorfluorescein diacetate (DCFH-DA) assay, respectively, for 12 or 24 h incubation after radiation. The optimal radiation dose and post-radiation incubation period were determined to be 8 Gy and 12 h. CCK-8 activity detection showed that the cell activity was 77.85% ( < 0.05, IC = 55.67 μg/mL). The apoptotic rate was the lowest (4.32%) at 200 μg/mL of PT-A-1 towards HaCaT cells. ROS production was the most effectively suppressed by 200 μg/mL PT-A-1 towards HaCaT cells. The persimmon tannin- gel composite has good radioprotective effect, and which will facilitate its clinic application as a potential natural anti-radiation agent in future.
柿单宁（L.f [山榄科]提取物）和 凝胶提取物（L.）Burm.f. [天门冬科]）被称为抗辐射剂。然而，柿单宁-凝胶复合物的辐射抗性尚不确定。为了研究柿单宁-凝胶复合物在细胞水平上对抗电离辐射的能力。将 HaCaT（人表皮角质形成细胞）细胞用 PT-A-1（柿单宁和 凝胶的质量比为 2:1）或单一成分（柿单宁或 凝胶）在不同浓度（0、50、100、200、400、800μg/mL）预处理。对照组：无 HaCaT 细胞的培养基），然后用 X 射线照射（辐射剂量：4、8、12、16 和 20Gy）。用 CCK-8 法、Hoechst 33258 染色/流式细胞术和 2'，7'-二氯荧光素二乙酸酯（DCFH-DA）法分别分析细胞活力、细胞凋亡和辐射诱导的细胞内活性氧（ROS）生成，辐射后 12 或 24 h 孵育。确定最佳辐射剂量和辐射后孵育时间分别为 8Gy 和 12h。CCK-8 活性检测显示细胞活性为 77.85%（<0.05，IC=55.67μg/mL）。PT-A-1 浓度为 200μg/mL 时，HaCaT 细胞的凋亡率最低（4.32%）。200μg/mL 的 PT-A-1 对 HaCaT 细胞中 ROS 的产生抑制最有效。柿单宁-凝胶复合物具有良好的辐射防护作用，这将有助于其在未来作为一种有潜力的天然抗辐射剂在临床上的应用。
