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利用通过开环易位聚合(ROMP)途径得到的抗菌聚合物检测细菌。

Detection of bacteria using antimicrobial polymer derived via ring-opening metathesis (romp) pathway.

作者信息

Süer N Ceren, Arasoğlu Tülin, Cankurtaran Hüsnü, Okutan Mustafa, Gallei Markus, Eren Tarik

机构信息

Department of Chemistry, Faculty of Arts and Science, Yıldız Technical University, İstanbul Turkey.

Department of Molecular Biology and Genetics, Faculty of Arts and Science, Yıldız Technical University, İstanbul Turkey.

出版信息

Turk J Chem. 2021 Aug 27;45(4):986-1003. doi: 10.3906/kim-2012-14. eCollection 2021.

Abstract

There is growing interest in the detection of bacteria in consumables, for example, in the food and water sectors. In this study, the aim was to produce a polymer-based bacteria biosensor via ROMP (ring opening metathesis polymerization). In the first part of the study, block and random copolymers were synthesized, and their biocidal activities were tested on the glass surface. Interdigitated electrode arrays coated with the polymers possessing the highest activity were used to screen the affinity towards different bacterial strains by monitoring impedance variations in real-time. The polymer-coated electrode could detect gram-positive and gram-negative bacteria strains at a concentration of 10 cfu/mL. The results show that ROMP-based polymer offers bacterial detection and can be used in developing biosensor devices for efficiently detecting pathogenic bacteria.

摘要

人们对检测消费品中的细菌越来越感兴趣,例如在食品和水领域。在本研究中,目标是通过开环易位聚合(ROMP)制备一种基于聚合物的细菌生物传感器。在研究的第一部分,合成了嵌段共聚物和无规共聚物,并在玻璃表面测试了它们的杀菌活性。使用涂覆有活性最高的聚合物的叉指电极阵列,通过实时监测阻抗变化来筛选对不同细菌菌株的亲和力。聚合物涂覆电极能够检测浓度为10 cfu/mL的革兰氏阳性和革兰氏阴性细菌菌株。结果表明,基于ROMP的聚合物可用于细菌检测,并可用于开发高效检测病原菌的生物传感器装置。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/65eb/8517495/938096586061/turkjchem-45-986-sch001.jpg

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