序列（dC-dA）n×（dG-dT）n在负超螺旋质粒中形成左手Z-DNA。
The sequence (dC-dA)n X (dG-dT)n forms left-handed Z-DNA in negatively supercoiled plasmids.
作者信息
Nordheim A, Rich A
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1821-5. doi: 10.1073/pnas.80.7.1821.
Abstract
Z-DNA-specific antibodies have been used to demonstrate the formation of left-handed Z-DNA in sequences of (dC-dA)32 X (dG-dT)32 in negatively supercoiled plasmids. Z-DNA was found to form at physiological negative superhelical densities (sigma = -0.05) in the absence of high salt concentration or chemical modifications. The binding site of the antibody to the DNA sequences was demonstrated by crosslinking experiments. (dC-dA)n X (dG-dT)n sequences are widespread in eukaryotic DNA, and possible roles for such Z elements in chromatin activation or genome rearrangements are suggested.
摘要
Z-DNA特异性抗体已被用于证明在负超螺旋质粒中(dC-dA)32×(dG-dT)32序列中左手螺旋Z-DNA的形成。在没有高盐浓度或化学修饰的情况下，发现Z-DNA在生理负超螺旋密度（σ = -0.05）时形成。通过交联实验证明了抗体与DNA序列的结合位点。(dC-dA)n×(dG-dT)n序列广泛存在于真核生物DNA中，并提示了此类Z元件在染色质激活或基因组重排中的可能作用。
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