青钱柳叶多糖对糖尿病大鼠脾脏损伤的抗氧化及免疫调节作用。
Antioxidative and immunological effects of Cyclocarya paliurus polysaccharides on the spleen injury of diabetic rat.
机构信息
Medical College, Pingdingshan University, Pingdingshan 467000, China.
Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
J Tradit Chin Med. 2021 Oct;41(5):739-746. doi: 10.19852/j.cnki.jtcm.20210203.001.
OBJEVTIVE
To investigate the effects of Cyclocarya paliurus (C. paliurus) polysaccharides on the spleen injury of diabetic rats.
METHODS
Animals were divided into 6 groups, including normal group, model group, control group, low-dose group of C. paliurus polysaccharides treatment, middle-dose group of C. paliurus polysaccharides treatment and high-dose group of C. paliurus polysaccharides treatment. Histological analysis of spleen was analyzed using hematoxilin and eosin. Levels of biological parameters and anti-oxidative enzymes were determined by spectrophotometry. Interleukin-7 (IL-7) and IL-10 were measured by enzyme-linked immunosorbent assay.
RESULTS
Compared with that of model group, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase level increased 78.63% (P < 0.05), 51.76% (P < 0.05), 2.95 times (P < 0.01) and 41.11% (P < 0.05) in the high-dose group of C. paliurus polysaccharides treatment, respectively. IL-7 and IL-10 increase 1.66 (P < 0.01) and 1.21 times (P < 0.01) in the high-dose group of C. paliurus polysaccharides treatment, respectively.
CONCLUSION
It is suggested that C. paliurus polysaccharides may play a protecting role for spleen injury of diabetic rats by enhancing the antioxidative ability and evaluating the immunity.
目的
研究青钱柳多糖对糖尿病大鼠脾脏损伤的影响。
方法
将动物分为 6 组，分别为正常组、模型组、对照组、青钱柳多糖低剂量组、青钱柳多糖中剂量组和青钱柳多糖高剂量组。采用苏木精-伊红染色法对脾脏进行组织学分析。通过分光光度法测定生物参数和抗氧化酶的水平。采用酶联免疫吸附试验测定白细胞介素-7（IL-7）和白细胞介素-10（IL-10）。
结果
与模型组相比，青钱柳多糖高剂量组超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶水平分别增加了 78.63%（P<0.05）、51.76%（P<0.05）、2.95 倍（P<0.01）和 41.11%（P<0.05）。IL-7 和 IL-10 分别增加了 1.66 倍（P<0.01）和 1.21 倍（P<0.01）。
结论
青钱柳多糖可能通过增强抗氧化能力和免疫评估来发挥对糖尿病大鼠脾脏损伤的保护作用。
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