School of Basic Medicine and Biological Science, Soochow University, Suzhou, 215123, People's Republic of China.
Jiangsu Fisheries Technology Promotion Center, Nanjing, 210036, People's Republic of China.
Fish Physiol Biochem. 2021 Dec;47(6):2015-2025. doi: 10.1007/s10695-021-01028-2. Epub 2021 Oct 28.
To reveal the impact of dietary fiber (DF) on the bile acid (BA) profiles of fish, yellow catfish (Pelteobagrus fulvidraco) were fed a diet containing 300 g kg dextrin (CON diet, control) or pectin (a type of soluble DF, PEC diet) for 7 days, and then the BA profiles were analyzed by UHPLC-MS/MS. A total of 26 individuals of BAs were detected in the fish body, with 8, 10, 14, and 22 individuals of BAs detected in the liver, serum, bile, and hindgut digesta, respectively. The conjugated BAs (CBAs) of fish were dominated by taurine CBAs (TCBAs). The concentrations of free BAs (FBAs) and the value of FBAs/CBAs in the bile of fish fed the PEC diet were nearly 5 and 7 times higher, respectively than those in fish fed the CON diet. The value of glycine CBAs/TCBAs in the liver, serum and bile of fish fed the PEC diet was significantly lower, and in the hindgut digesta was higher than that of fish fed the CON diet (P < 0.05). These results suggested that dietary pectin greatly changed the BA profiles of Pelteobagrus fulvidraco, attributed to inhibition of reabsorption of BAs. Therefore, attention should be paid to the impact on BA homeostasis when replacing fishmeal with DF-rich plant ingredients in the fish diet.
为了揭示膳食纤维（DF）对鱼类胆汁酸（BA）谱的影响，将黄颡鱼（Pelteobagrus fulvidraco）分别投喂含 300 g kg 糊精（CON diets，对照）或果胶（一种可溶性 DF）的日粮 7 天，然后采用 UHPLC-MS/MS 分析 BA 谱。在鱼体中检测到 26 种 BA，在肝脏、血清、胆汁和后肠消化物中分别检测到 8、10、14 和 22 种 BA。鱼中的结合型 BA（CBAs）主要为牛磺酸结合型 BA（TCBAs）。与 CON 日粮相比，饲喂 PEC 日粮的鱼胆汁中的游离 BA（FBAs）浓度和 FBAs/CBAs 值分别高近 5 倍和 7 倍。饲喂 PEC 日粮的鱼的肝脏、血清和胆汁中的甘氨酸结合型 BA/TCBAs 值显著降低，而后肠消化物中的值则高于 CON 日粮（P<0.05）。这些结果表明，日粮果胶极大地改变了黄颡鱼的 BA 谱，这归因于 BA 重吸收的抑制。因此，在用富含 DF 的植物成分替代鱼粉配制鱼类日粮时，应注意对 BA 平衡的影响。
