通过用登革病毒颗粒刺激外周血单个核细胞在体外产生人抗体分泌细胞。

In Vitro Generation of Human Antibody-Secreting Cells Through the Stimulation of PBMCs with Dengue Virus Particles.

作者信息

Bonezi Vivian, Cataneo Allan Henrique Depieri, Wowk Pryscilla Fanini, Silveira Eduardo L V

机构信息

Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.

Laboratório de Virologia Molecular, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, PR, Brazil.

出版信息

Methods Mol Biol. 2022;2409:235-244. doi: 10.1007/978-1-0716-1879-0_16.

DOI:10.1007/978-1-0716-1879-0_16

PMID:34709646

Abstract

The Dengue pathophysiology has had several aspects determined over the years. However, some points remain elusive, such as the metabolic factors that regulate the massive B cell differentiation into antibody-secreting cells observed in Dengue patients. In this chapter, we describe an in vitro method capable of mimicking this Dengue-induced cell expansion. More specifically, this approach allows dengue virus-stimulated peripheral blood mononuclear cells (PBMCs) from healthy individuals to enhance the frequency of phenotypical and functional antibody-secreting cells (ASCs) after 7 days of culture. A manuscript recently published by Bonezi and colleagues displays results generated through this methodology.

摘要

多年来，登革热的病理生理学的多个方面已被确定。然而，一些问题仍然难以捉摸，比如在登革热患者中观察到的调节大量B细胞分化为抗体分泌细胞的代谢因素。在本章中，我们描述了一种能够模拟这种登革热诱导的细胞扩增的体外方法。更具体地说，这种方法使来自健康个体的登革热病毒刺激的外周血单核细胞（PBMC）在培养7天后增加表型和功能性抗体分泌细胞（ASC）的频率。Bonezi及其同事最近发表的一篇论文展示了通过这种方法产生的结果。