Ding Xiudong, Zhou Jing, Chai Yinghui, Yan Zengkui, Liu Xin, Dong Yueming, Mei Xue, Jiang Ying, Lei Hong
8th Medical Center of PLA General Hospital, China.
Hebei North University, China.
Microbes Infect. 2022 Mar;24(2):104893. doi: 10.1016/j.micinf.2021.104893. Epub 2021 Oct 25.
There is an abundant link between the gut microbiota and human health and it plays a critical role in the clinic. It is recognized that microbial dysregulation contributes to the pathogenesis of tuberculosis (TB) but the underlying mechanisms remain unclear. In this study, we investigated the association of gut microbiome composition with TB as well as its possible roles in the development of this disease.
Fecal samples were collected from 10 TB patients and 20 healthy control samples. DNA extracted from fecal samples was subjected to 16S rDNA gene sequencing analysis on the Illumina MiSeq platform.
Compared with healthy control samples, the gut microbiome of patients with TB was characterized by the decreased Alpha diversity. Perhaps, the decrease of microbial diversity which results in microbial dysregulation is the reason for clinical patients with more symptoms. The PTB group showed the most unique microbiota by higher abundance of Bifidobacteriaceae, Bifidobacteriales, Coriobacteriaceae, Coriobacteriales, Actinobacteria, Caulobacteraceae, Phyllobacteriaceae, Rhizobiales, Burkholderiaceae, Burkholderiaceae. Inflammatory status in PTB patients may be associated with the increased abundance of Clostridia and decreased abundance of Prevotella. We found that the abundance of Solobacterium and Actinobacteria was higher in the patients. There were 4 significant differences (p < 0.05) in the two groups which belonged to four metabolic categories, including endocytosis, phosphotransferase system (PTS), toluene degradation, and amoebiasis.
We applied the approach of metagenomic sequencing to characterize the features of gut microbiota in PTB patients. The present study provided a detailed analysis of the characterization of the gut microbiota in patients based on the clinic. According to the metagenome analysis, our results indicated that the gut microbiota in PTB patients was significantly different from healthy control samples as characterized by the bacteria and metabolic pathway. The richness of the gut microbiota in patients was revealed. It was hypothesized that the above-mentioned changes of the gut microbiota could exert an impact on the development of PTB through the downstream regulation of the immune status of the host by way of the gut-lung axis.
肠道微生物群与人类健康之间存在着丰富的联系，并且在临床上发挥着关键作用。人们认识到微生物失调会导致结核病（TB）的发病机制，但潜在机制仍不清楚。在本研究中，我们调查了肠道微生物群组成与结核病的关联及其在该疾病发展中的可能作用。
从10名结核病患者和20名健康对照样本中收集粪便样本。从粪便样本中提取的DNA在Illumina MiSeq平台上进行16S rDNA基因测序分析。
与健康对照样本相比，结核病患者的肠道微生物群具有Alpha多样性降低的特征。也许，微生物多样性的降低导致微生物失调，这是临床患者出现更多症状的原因。肺结核（PTB）组显示出最独特的微生物群，双歧杆菌科、双歧杆菌目、棒状杆菌科、棒状杆菌目、放线菌、柄杆菌科、叶杆菌科、根瘤菌目、伯克霍尔德菌科、伯克霍尔德菌科的丰度更高。PTB患者的炎症状态可能与梭菌丰度增加和普雷沃菌丰度降低有关。我们发现患者中解脲芽孢杆菌和放线菌的丰度较高。两组之间在四个代谢类别中存在4个显著差异（p < 0.05），包括内吞作用、磷酸转移酶系统（PTS）、甲苯降解和阿米巴病。
我们应用宏基因组测序方法来表征PTB患者肠道微生物群的特征。本研究基于临床对患者肠道微生物群的特征进行了详细分析。根据宏基因组分析，我们的结果表明，PTB患者的肠道微生物群与健康对照样本存在显著差异，其特征在于细菌和代谢途径。揭示了患者肠道微生物群的丰富度。据推测，上述肠道微生物群的变化可能通过肠道 - 肺轴对宿主免疫状态的下游调节，对PTB的发展产生影响。
