Schrodi Valentin, Neunaber Claudia, Bundkirchen Katrin, Ye Weikang, Jiang Zhida, Petri Maximilian, Krettek Christian, Noack Sandra
Trauma Department, Hannover Medical School, 30625 Hannover, Germany.
Department of Urology and Urooncology, Clinic of Braunschweig, 38126 Braunschweig, Germany.
Stem Cells Int. 2021 Oct 19;2021:6864988. doi: 10.1155/2021/6864988. eCollection 2021.
Mesenchymal stem cells play an important role in regenerative medicine due to their capability of self-renewal and multipotent differentiation. For research or clinical application, bone marrow aspirates are harvested during elective surgeries to isolate MSCs. If an immediate purification of the MSCs is not possible, the bone marrow must be stored. Therefore, the aim of this study was to investigate possible differences of stem cell characteristics regarding the self-renewal capability, the adipogenic, chondrogenic, and osteogenic differentiation, and the expression of surface antigens after different storage conditions of the bone marrow aspirates. Three groups were analysed: the first group was purified immediately after harvesting, the other two groups were processed after they were stored 18 to 24 hours at 22°C (room temperature) or at 4°C. Comparisons between the groups were performed using the Kruskal-Wallis test for nonparametric data. The final results showed no significant difference between the different storage conditions. Therefore, storage of bone marrow aspirates for 18 to 24 hours at room temperature or 4°C is possible without loss of stem cell characteristics.
间充质干细胞因其自我更新和多能分化能力在再生医学中发挥着重要作用。对于研究或临床应用，在择期手术期间采集骨髓抽吸物以分离间充质干细胞。如果无法立即对间充质干细胞进行纯化，则必须储存骨髓。因此，本研究的目的是调查骨髓抽吸物在不同储存条件下，间充质干细胞在自我更新能力、成脂、成软骨和成骨分化以及表面抗原表达方面的干细胞特性可能存在的差异。分析了三组：第一组在采集后立即纯化，另外两组在22°C（室温）或4°C储存18至24小时后进行处理。使用Kruskal-Wallis检验对非参数数据进行组间比较。最终结果表明不同储存条件之间没有显著差异。因此，骨髓抽吸物在室温或4°C下储存18至24小时是可行的，不会丧失干细胞特性。
