Hayden Julia, Fang-Yen Christopher
Department of Bioengineering, University of Pennsylvania.
MicroPubl Biol. 2021 Oct 25;2021. doi: 10.17912/micropub.biology.000488. eCollection 2021.
Ultracold storage is widely used to preserve genetic stocks. Standard cryopreservation methods for the nematode are vulnerable to refrigeration failures, which can result in the loss of stock viability due to freeze-thaw damage. In previous work our laboratory developed a method for cryopreserving worms in a dehydrated form that remains viable after multiple freeze-thaw cycles. However, strains preserved in this manner can be recovered only once from each cryopreservation tube. Here we describe a cryopreservation method in which are dehydrated in a granular medium (cornmeal) prior to freezing. To recover worms, a small fraction (~1%) of the medium may be removed with the remainder returned to cold storage. Our improved cryopreservation method is not only resistant to refrigeration failures but also greatly increases the number of recoveries per tube compared to current methods.
超低温保存被广泛用于保存基因库。线虫的标准冷冻保存方法容易受到制冷故障的影响，这可能会因冻融损伤而导致库存活力丧失。在之前的工作中，我们实验室开发了一种以脱水形式冷冻保存线虫的方法，这种线虫在多次冻融循环后仍能存活。然而，以这种方式保存的菌株每次只能从每个冷冻保存管中复苏一次。在这里，我们描述了一种冷冻保存方法，即线虫在冷冻前在颗粒介质（玉米粉）中脱水。为了复苏线虫，可以取出一小部分（约1%）的介质，其余部分则返回冷藏。我们改进的冷冻保存方法不仅能抵抗制冷故障，而且与目前的方法相比，还大大增加了每个管子的复苏次数。
