Department of Geriatrics, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, P.R. China.
Department of Emergency, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510000, P.R. China.
Mol Med Rep. 2021 Dec;24(6). doi: 10.3892/mmr.2021.12514. Epub 2021 Oct 29.
Long‑term hypertension leads to alterations in the structure and function of blood vessels, and abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) are important factors for these changes. Linalool is a natural compound extracted from plants. The present study aimed to explore the role and underlying mechanism of linalool in the physiological behavior of VSMCs. Angiotensin II (Ang II) was utilized to treat VSMCs, and MTT and western blotting assays were then employed to detect the effect of linalool on the induced proliferation and migration of VSMCs. The target gene of linalool was predicted by the SwissTargetPrediction website, and its expression level in VSMCs was determined using reverse transcription‑quantitative PCR and western blotting. Next, the role of the target gene in the physiological behavior of VSMCs treated with linalool was examined, and the signaling pathway was explored. The results revealed that the proliferation and migration of VSMCs treated with Ang II were significantly promoted, and linalool could alleviate these effects in a dose‑dependent manner. Cholinergic receptor muscarinic 3 (CHRM3), as a predicted target, was found to be highly expressed in Ang II‑induced VSMCs, and CHRM3 overexpression could prevent the inhibitory effect of linalool on cell proliferation and migration. In addition, its overexpression caused an increase in the expression of proteins related to the MAPK signaling pathway. In conclusion, linalool inhibited the proliferation and migration of Ang II‑induced VSMCs and blocked the MAPK signaling pathway by downregulating CHRM3.
长期高血压可导致血管结构和功能发生改变，血管平滑肌细胞（VSMCs）异常增殖和迁移是这些变化的重要因素。芳樟醇是一种从植物中提取的天然化合物。本研究旨在探讨芳樟醇在 VSMCs 生理行为中的作用及其潜在机制。用血管紧张素 II（Ang II）处理 VSMCs，然后采用 MTT 和 Western blot 检测芳樟醇对 Ang II 诱导的 VSMCs 增殖和迁移的影响。通过 SwissTargetPrediction 网站预测芳樟醇的靶基因，并采用逆转录-定量 PCR 和 Western blot 检测其在 VSMCs 中的表达水平。接下来，检测靶基因在芳樟醇处理的 VSMCs 生理行为中的作用，并探讨其信号通路。结果表明，用 Ang II 处理可显著促进 VSMCs 的增殖和迁移，而芳樟醇可呈剂量依赖性减轻这些作用。作为预测靶标的毒蕈碱型乙酰胆碱受体 M3（CHRM3）在 Ang II 诱导的 VSMCs 中表达水平较高，CHRM3 过表达可阻止芳樟醇对细胞增殖和迁移的抑制作用。此外，其过表达导致与 MAPK 信号通路相关的蛋白表达增加。综上所述，芳樟醇通过下调 CHRM3 抑制 Ang II 诱导的 VSMCs 的增殖和迁移，并阻断 MAPK 信号通路。
