Oil and Gas Field Applied Chemistry Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, Southwest Petroleum University, Sichuan, China.
Research Institute of Industrial Hazardous Waste Disposal and Resource Utilization, Southwest Petroleum University, Sichuan, China.
Luminescence. 2022 Jan;37(1):108-117. doi: 10.1002/bio.4151. Epub 2021 Nov 13.
In this paper, a novel rapid, highly selective and sensitive Pb fluorescent probe (E)-N'-((2-(4'-(diphenylamino)-[1,1'-biphenyl]-4-yl)-2H-1,2,3-triazol-4-yl)methylene) (DBTBH) was synthesized. The probe DBTBH not only exhibited more excellent selectivity and sensitivity to Pb detection compared with other analytes (include metal ions and anions) in H O:THF solution (v:v = 9:1, 10 mM Tris-HCl, 1 mM KI, pH 7.4), but also had excellent optical properties such as aggregation-induced emission enhancement (AIEE) and intramolecular charge transfer (ICT). Detection limit of the probe DBTBH towards Pb was 4.49 × 10 M. The possible mechanism was verified by H NMR titration and HR-MS. Furthermore, the successful detection of Pb by DBTBH in real water samples and HeLa cells indicated that DBTBH has great potential for selective recognition of Pb in the natural environment and biological systems. These findings will provide a promising new idea for designing better Pb fluorescent probes in the future.
本文合成了一种新型快速、高选择性和高灵敏度的 Pb 荧光探针（E）-N'-((2-(4'-(二苯胺基)-[1,1'-联苯]-4-基)-2H-1,2,3-三唑-4-基)亚甲基)(DBTBH)。与 H2O:THF 溶液（v:v=9:1, 10 mM Tris-HCl, 1 mM KI, pH 7.4）中的其他分析物（包括金属离子和阴离子）相比，探针 DBTBH 不仅对 Pb 的检测具有更优异的选择性和灵敏度，而且具有出色的光学性质，如聚集诱导发射增强（AIEE）和分子内电荷转移（ICT）。探针 DBTBH 对 Pb 的检测限为 4.49 × 10-7M。通过 H NMR 滴定和 HR-MS 验证了可能的机制。此外，DBTBH 在实际水样和 HeLa 细胞中成功检测到 Pb，表明 DBTBH 具有在自然环境和生物系统中选择性识别 Pb 的巨大潜力。这些发现将为未来设计更好的 Pb 荧光探针提供有前景的新思路。
