Harbin Institute of Technology, Harbin 150001, P. R. China.
Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, P. R. China.
Anal Chem. 2021 Nov 9;93(44):14820-14827. doi: 10.1021/acs.analchem.1c03713. Epub 2021 Oct 29.
Photoacoustic imaging reveals great potential for the study of individual cells due to the rich imaging contrast for both label-free and labeled cells. However, previously reported photoacoustic imaging flow cytometry configuration suffers from inadequate imaging quality and challenge to distinguish multiple cells. In order to solve such issues, we propose a novel acoustic standing wave aided multiparametric photoacoustic imaging flow cytometry (MPAFC) system. The acoustic standing wave is introduced to improve the imaging quality and speed. Multispectral illumination along with cell geometry, photoacoustic amplitude, and acoustic frequency spectrum enables the proposed system to precisely identify multiple types of cells with one scanning. On the basis of the identification, elimination of melanoma cells, and targeted labeled glioma cells have been performed with an elimination efficiency of >95%. Additionally, the MPAFC system is able to image and capture melanoma cells at a lowest concentration of 100 cells mL in pure blood. Current results suggest that the proposed MPAFC may provide a precise and efficient tool for cell detection, manipulation, and elimination in both fundamental and clinical studies.
光声成像是一种很有前途的研究方法，因为它对无标记和标记细胞都具有丰富的成像对比度。然而，以前报道的光声成像流式细胞术配置存在成像质量不足和难以区分多个细胞的挑战。为了解决这些问题，我们提出了一种新的声学驻波辅助多参数光声成像流式细胞术（MPAFC）系统。引入声学驻波来提高成像质量和速度。多光谱照明以及细胞几何形状、光声幅度和声波频谱使该系统能够通过一次扫描精确识别多种类型的细胞。在此基础上，我们对黑素瘤细胞进行了消除，对靶向标记的神经胶质瘤细胞的消除效率>95%。此外，该 MPAFC 系统能够在纯血中以最低 100 个细胞/mL 的浓度对黑素瘤细胞进行成像和捕获。目前的结果表明，所提出的 MPAFC 可能为基础和临床研究中的细胞检测、操作和消除提供一种精确和高效的工具。
