Sun Aihui, Li Yaxi, Zhu Pengfei, He Xiaoliang, Jiang Zhilong, Kong Yan, Liu Cheng, Wang Shouyu
Department of Optoelectronic Information Science and Engineering, School of Science, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Radiology Department, Jiangnan University Medical Center, Wuxi, Jiangsu, 214122, China.
Biomed Opt Express. 2023 Sep 14;14(10):5199-5207. doi: 10.1364/BOE.504863. eCollection 2023 Oct 1.
In this work, we design multi-parameter phase imaging flow cytometry based on dual-view transport of intensity (MPFC), which integrates phase imaging and microfluidics to a microscope, to obtain single-shot quantitative phase imaging on cells flowing in the microfluidic channel. The MPFC system has been proven with simple configuration, accurate phase retrieval, high imaging contrast, and real-time imaging and has been successfully employed not only in imaging, recognizing, and analyzing the flowing cells even with high-flowing velocities but also in tracking cell motilities, including rotation and binary rotation. Current results suggest that our proposed MPFC provides an effective tool for imaging and analyzing cells in microfluidics and can be potentially used in both fundamental and clinical studies.
在本研究中，我们基于强度双视图传输设计了多参数相成像流式细胞术（MPFC），该技术将相成像和微流体技术集成到显微镜中，以对微流体通道中流动的细胞进行单次定量相成像。MPFC系统已被证明具有结构简单、相位恢复准确、成像对比度高和实时成像的特点，不仅成功应用于对即使具有高流速的流动细胞进行成像、识别和分析，还用于跟踪细胞运动，包括旋转和双旋转。目前的结果表明，我们提出的MPFC为微流体中的细胞成像和分析提供了一种有效工具，并且可能在基础研究和临床研究中都有应用。
