Department of Endodontics and Molecular Microbiology Laboratory, Faculty of Dentistry, Grande Rio University, Rio de Janeiro, Brazil.
Department of Dental Research, Faculty of Dentistry, Iguaçu University (UNIG), Nova Iguaçu, Brazil.
Int Endod J. 2022 Mar;55 Suppl 1:46-71. doi: 10.1111/iej.13656. Epub 2021 Nov 16.
Endodontic microbiology deals with the study of the microbial aetiology and pathogenesis of pulpal and periradicular inflammatory diseases. Research in endodontic microbiology started almost 130 years ago and since then has mostly focussed on establishing and confirming the infectious aetiology of apical periodontitis, identifying the microbial species associated with the different types of endodontic infections and determining the efficacy of treatment procedures in eradicating or controlling infection. Diverse analytical methods have been used over the years, each one with their own advantages and limitations. In this review, the main features and applications of the most used technologies are discussed, and advice is provided to improve study designs in order to properly address the scientific questions and avoid setbacks that can compromise the results. Finally, areas of future research are described.
牙髓微生物学研究牙髓和根尖周炎症性疾病的微生物病因和发病机制。牙髓微生物学的研究始于近 130 年前，从那时起，主要集中于确定根尖周炎的感染病因，鉴定与不同类型牙髓感染相关的微生物种类，并确定治疗程序根除或控制感染的效果。多年来已经使用了多种分析方法，每种方法都有其自身的优点和局限性。在这篇综述中，讨论了最常用技术的主要特点和应用，并提供了改进研究设计的建议，以便正确解决科学问题，避免可能影响结果的挫折。最后，描述了未来的研究领域。
