College of Medicine and Dentistry, James Cook University, 1/14-88 McGregor Rd, Building D1, 2nd Floor, Campus Smithfield, Smithfield, Cairns, QLD, 4878, Australia.
School of Medicine and Dentistry, Griffith University, Gold Coast, Queensland, Australia.
Clin Oral Investig. 2024 Feb 8;28(2):139. doi: 10.1007/s00784-024-05544-2.
This study aimed to describe the effects of two single-file systems on the diversity of the endodontic microbiome of teeth with primary asymptomatic apical periodontitis.
The root canals from single-rooted teeth with apical periodontitis were prepared using either the Reciproc Blue (RB) or the XP-endo Shaper (XPS) instrument system. The latter was followed by a supplementary step with the XP-endo Finisher (XPF) instrument. For irrigation, 5.25% sodium hypochlorite was used. Root canal samples were taken at the baseline (S1), after preparation (S2), and after the supplementary step (S3). DNA was extracted and subjected to high-throughput sequencing using the MiSeq Illumina platform.
Samples from 10 teeth from the RB and 7 from the XPS group were subjected to DNA sequencing. Initial samples differed significantly from post-preparation samples in bacterial diversity, with no significant difference when comparing the two instrument systems. The most dominant phyla in S2 were Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, and Actinobacteria. The same phyla were found to dominate baseline samples and samples taken after using XPF, but with differences in the ranking of the most dominant ones. At the genus level, the most dominant genera identified after RB instrumentation were Bacteroidaceae [G-1], Fusobacterium, and Staphylococcus, while the most dominant genera after XPS instrumentation were Fusobacterium and Porphyromonas. These genera were also dominant in the initial samples.
Both treatment protocols had measurable effects on the root canal microbial diversity, with no significant differences between them. Most of the dominant taxa involved in the primary infection and probably in the aetiology of apical periodontitis were eliminated or substantially reduced.
The most dominant taxa that persisted after instrumentation were Fusobacterium, Porphyromonas, Staphylococcus, and Bacteroidaceae [G-1].
本研究旨在描述两种单文件系统对原发性无症状根尖周炎牙齿根管内微生物多样性的影响。
使用 Reciproc Blue（RB）或 XP-endo Shaper（XPS）仪器系统对根尖周炎的单根牙的根管进行预备。后者随后用 XP-endo Finisher（XPF）仪器进行补充步骤。冲洗时使用 5.25%次氯酸钠。在基线（S1）、预备后（S2）和补充步骤后（S3）采集根管样本。提取 DNA 并使用 MiSeq Illumina 平台进行高通量测序。
对来自 RB 组的 10 个牙齿样本和 XPS 组的 7 个牙齿样本进行了 DNA 测序。初始样本的细菌多样性与预备后样本有显著差异，而两种仪器系统之间无显著差异。S2 中最主要的菌门为拟杆菌门、变形菌门、厚壁菌门、梭杆菌门和放线菌门。在基线样本和使用 XPF 后的样本中也发现了相同的菌门，但优势菌门的排名有所不同。在属水平上，在 RB 器械处理后鉴定的最主要的属为拟杆菌科[G-1]、梭杆菌属和葡萄球菌属，而在 XPS 器械处理后最主要的属为梭杆菌属和卟啉单胞菌属。这些属在初始样本中也占主导地位。
两种治疗方案均对根管微生物多样性产生了可测量的影响，但它们之间无显著差异。涉及原发性感染和根尖周炎发病机制的大多数优势类群被消除或大量减少。
在器械处理后仍然存在的最主要的类群是梭杆菌属、卟啉单胞菌属、葡萄球菌属和拟杆菌科[G-1]。
