Office of Regulatory Science, Center for Food Safety and Applied Nutrition (CFSAN), Food and Drug Administration, College Park, Maryland 20740, United States.
J Agric Food Chem. 2021 Nov 17;69(45):13331-13338. doi: 10.1021/acs.jafc.1c02156. Epub 2021 Oct 29.
The xMAP food allergen detection assay (xMAP FADA) is an advanced multiplex immunoassay with multiple antibodies for each of 15 target food allergens and gluten, allowing for signal confirmation and antigenic profiling to occur in a single analysis. Botanicals used as spices are complex matrices for allergen analysis because they can exhibit inherent cross-reactivity with antibodies employed by the assays. Preliminary examinations of botanicals revealed chili peppers to have notably high levels of cross-reactivity with Brazil nut and hazelnut antibody bead sets in the xMAP FADA. This in-depth investigation of 29 pre-ground and whole chili peppers indicated Brazil nut and hazelnut cross-reactivity to be consistent among most members of genus, although cross-reactive signals generated by chili peppers were distinguishable from signals indicative of target allergen detection. Using the requirements that complementary antibodies used in the assay generated positive responses and that the various secondary end points were characteristic of the target analytes, xMAP FADA reactivity to chilis of the genus was categorized as cross-reactivity instead of confirmed detection of target allergenic foods.
xMAP 食物过敏原检测分析（xMAP FADA）是一种先进的多重免疫分析方法，每个分析中包含 15 种目标食物过敏原和谷蛋白的多种抗体，允许在单个分析中进行信号确认和抗原分析。作为香料使用的植物性食物是过敏原分析的复杂基质，因为它们可能与分析中使用的抗体表现出固有交叉反应性。对植物性食物的初步检查表明，辣椒与 xMAP FADA 中的巴西坚果和榛子抗体珠组有明显的高交叉反应性。对 29 种研磨和整粒辣椒的深入调查表明，巴西坚果和榛子的交叉反应性在大多数属的成员中是一致的，尽管辣椒产生的交叉反应性信号与指示目标过敏原检测的信号不同。使用分析中使用的互补抗体产生阳性反应的要求，以及各种次要终点是目标分析物的特征，xMAP FADA 对辣椒属的反应被归类为交叉反应性，而不是对目标过敏原食物的确认检测。
