Laboratório de Plantas Medicinais LAPLAMED, Programa de Pós-Graduação em Ciências Farmacêuticas CiPharma, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, 35400-000, Brazil.
Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, 35400-000, Brazil.
J Ethnopharmacol. 2022 Feb 10;284:114778. doi: 10.1016/j.jep.2021.114778. Epub 2021 Oct 28.
Coffea arabica is commonly known for its cardiotonic and neurotonic activities, but in some places' folk medicine, like in Arabia and Africa, C. arabica is used to treat headache, migraine, the flu, anemia, oedema, asthenia, asthma, inflammation and wounds.
The aims were to evaluate if the aqueous extracts of Coffea arabica, prepared from beans with different degrees of roasting, and their main chemical constituents could exert an in vivo anti-gouty effect.
Coffea extracts were obtained from the beans of not roasted, light, medium and dark roasted coffee and from decaffeinated and traditional coffees and were prepared with water at 25°C and at 98°C. C57BL/6 mice were induced to gout by an injection of monosodium urate crystals and treated with coffee extracts at doses of 25, 75 and 225 mg/kg and their chemical constituents at a dose of 10 mg/kg. The antinociceptive and anti-inflammatory effects were evaluated.
Treatments with Coffea extracts prepared with water at 98°C were more effective to exert antinociceptive and anti-inflammatory activities than the ones prepared with water at 25°C. Caffeic and chlorogenic acids reduced hypernociception in animals when compared with negative control group (7.79 and 5.69 vs 18.53; P < 0.05 and P < 0.001, respectively), inhibited neutrophil migration (1.59 × 104 and 0.38 × 104 vs 9.47 × 104; P < 0.0001 both) and decreased pro-inflammatory cytokines concentration (IL-1β, IL-6 and TNF-α).
We have demonstrated that our treatments attenuated gout, and this effect could be attributed to a reducement in hypernociception, neutrophil migration and cytokines concentration. These results suggest coffee as a potential candidate for studies in acute gout therapy.
阿拉伯咖啡因其具有强心和神经兴奋作用而广为人知，但在某些地方的民间医学中，如在阿拉伯和非洲，阿拉伯咖啡被用于治疗头痛、偏头痛、流感、贫血、水肿、虚弱、哮喘、炎症和伤口。
本研究旨在评估不同烘焙程度的咖啡豆制备的阿拉伯咖啡的水提取物及其主要化学成分是否具有体内抗痛风作用。
从未烘焙、轻度烘焙、中度烘焙和深度烘焙的咖啡豆以及脱咖啡因咖啡和传统咖啡中提取咖啡提取物，用 25°C 和 98°C 的水制备。用尿酸单钠晶体注射诱导 C57BL/6 小鼠发生痛风，并以 25、75 和 225mg/kg 的剂量给予咖啡提取物及其化学组成物 10mg/kg 的剂量进行治疗。评估了镇痛和抗炎作用。
用 98°C 水制备的咖啡提取物比用 25°C 水制备的咖啡提取物更有效地发挥镇痛和抗炎作用。咖啡酸和绿原酸与阴性对照组相比，降低了动物的高敏反应（7.79 和 5.69 比 18.53；P<0.05 和 P<0.001），抑制了中性粒细胞迁移（1.59×104 和 0.38×104 比 9.47×104；均 P<0.0001），并降低了促炎细胞因子浓度（IL-1β、IL-6 和 TNF-α）。
我们已经证明，我们的治疗方法减轻了痛风，这种作用可能归因于降低了高敏反应、中性粒细胞迁移和细胞因子浓度。这些结果表明咖啡可能是急性痛风治疗研究的潜在候选药物。
