Key Laboratory of Animal Gennetics, Breeding and Reproducation of Shaanxi Province, Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, 712100, China.
Key Laboratory of Animal Gennetics, Breeding and Reproducation of Shaanxi Province, Laboratory of Animal Fat Deposition & Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, 712100, China.
Theriogenology. 2022 Jan 1;177:172-182. doi: 10.1016/j.theriogenology.2021.10.018. Epub 2021 Oct 20.
Artificial insemination (AI) is a proven breeding technology which has been widely used in pig reproduction. Low temperature can cause very serious damage to pig sperm below 15 °C and the situation is even more serious at lower temperature. Besides, the preservation of pig sperm is mainly carried out at 17 °C because of its outstanding performance in pig reproduction. However, the accumulation of a large amount of reactive oxygen species (ROS) during the preservation process is the main reason for the deterioration of sperm quality. In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of pig sperm at 17 °C, we compared them with the traditional diluent to study the sperm motility, the cumulative amount of ROS, the extent of sperm membrane damage, the sperm acrosome integrity, the sperm DNA damage and the activity of antioxidant enzymes (CAT, T-AOC, SOD, GSH-PX, MDA) to evaluate the effect of hydroxytyrosol on the sperm quality during storage. We used proteomics sequencing technology to monitor difference in sperm protein between the control samples and the addition of 120 μmol/L hydroxytyrosol samples (optimum concentration) after three days storage. Ultimately, we selected the control samples and the addition of 120 μmol/L hydroxytyrosol samples to test the effect of AI. The results of our research showed that during storage of pig sperm at 17 °C, the sperm quality and antioxidant capacity of the hydroxytyrosol-treated samples significantly improved (HT 120 μmol/L) (P < 0.05). Proteomics sequencing analysis proved that the addition of 120 μmol/L hydroxytyrosol treatment samples had potential value in improving sperm quality. The significant increase in sow pregnancy rate and piglet birth weight proved that hydroxytyrosol had important practical value in pig reproduction. Based on our results, we demonstrated that the addition of hydroxytyrosol to the diluent could improve the quality of pig sperm and the efficiency of AI.
人工授精（AI）是一种经过验证的繁殖技术，已广泛应用于猪的繁殖。低温会对 15°C 以下的猪精子造成非常严重的损害，温度越低情况越严重。此外，由于在猪繁殖中表现出色，猪精子的保存主要在 17°C 下进行。然而，在保存过程中大量活性氧（ROS）的积累是导致精子质量恶化的主要原因。在我们的研究中，通过在 17°C 下储存猪精子时在稀释剂中添加不同浓度的羟基酪醇，将其与传统稀释剂进行比较，研究了精子活力、ROS 的累积量、精子膜损伤程度、精子顶体完整性、精子 DNA 损伤和抗氧化酶的活性（CAT、T-AOC、SOD、GSH-PX、MDA），以评估羟基酪醇对储存期间精子质量的影响。我们使用蛋白质组学测序技术监测储存三天后对照组和添加 120μmol/L 羟基酪醇组（最佳浓度）之间精子蛋白的差异。最终，我们选择对照组和添加 120μmol/L 羟基酪醇组来测试 AI 的效果。我们的研究结果表明，在 17°C 下储存猪精子时，添加 120μmol/L 羟基酪醇处理组的精子质量和抗氧化能力显著提高（HT 120μmol/L）（P<0.05）。蛋白质组学测序分析证明，添加 120μmol/L 羟基酪醇处理组在提高精子质量方面具有潜在价值。母猪妊娠率和仔猪出生体重的显著提高证明了羟基酪醇在猪繁殖中具有重要的实际价值。基于我们的结果，我们证明了在稀释剂中添加羟基酪醇可以提高猪精子的质量和 AI 的效率。
