Zhang Haize, Chai Jiawen, Cao Chaoyue, Wang Xiaolin, Pang Weijun
Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang 712100, China.
Antioxidants (Basel). 2024 Apr 24;13(5):507. doi: 10.3390/antiox13050507.
Sperm quality is an important indicator to evaluate the reproduction ability of animals. Nicotinamide mononucleotide (NMN) participates in cell energy metabolism and reduces cell oxidative stress. However, the effect and regulatory mechanism of NMN on porcine sperm quality are still unknown. Here, 32 Landrace boars were randomly assigned to four groups ( = 8) and fed with different levels of NMN (0, 8, 16 or 32 mg/kg/d) for 9 weeks, and then serum and semen samples of the boars were collected to investigate the function and molecular mechanism of NMN in sperm quality. The results showed that the dietary NMN supplementation significantly increased sperm volume, density and motility ( < 0.05). Interestingly, NMN apparently improved the antioxidative indexes and increased the levels of testosterone ( < 0.05) in serum. Furthermore, NMN upregulated the protein levels of sirtuin 3 (SIRT3), antioxidation and oxidative phosphorylation (OXPHOS), but downregulated the protein levels of apoptosis in semen. Mechanically, NMN protected sperm from HO-induced oxidative stress and apoptosis through SIRT3 deacetylation. Importantly, the SIRT3-specific inhibitor 3-TYP attenuated the antioxidation and antiapoptosis of NMN in sperm. Therefore, NMN exerts antioxidation and antiapoptosis to improve boar sperm quality via the SIRT3 signaling pathway. Our findings suggest that NMN is a novel potential boar antioxidative feed additive to produce high-quality porcine semen.
精子质量是评估动物繁殖能力的重要指标。烟酰胺单核苷酸（NMN）参与细胞能量代谢并降低细胞氧化应激。然而，NMN对猪精子质量的影响及其调控机制仍不清楚。在此，将32头长白公猪随机分为四组（每组n = 8），分别饲喂不同水平的NMN（0、8、16或32 mg/kg/d），持续9周，然后采集公猪的血清和精液样本，以研究NMN在精子质量方面的作用及分子机制。结果表明，日粮中添加NMN显著增加了精液体积、密度和活力（P < 0.05）。有趣的是，NMN明显改善了抗氧化指标，并提高了血清中睾酮水平（P < 0.05）。此外，NMN上调了精液中沉默调节蛋白3（SIRT3）、抗氧化和氧化磷酸化（OXPHOS）的蛋白水平，但下调了凋亡蛋白水平。从机制上讲，NMN通过SIRT3去乙酰化保护精子免受H2O2诱导的氧化应激和凋亡。重要的是，SIRT3特异性抑制剂3-TYP减弱了NMN对精子的抗氧化和抗凋亡作用。因此，NMN通过SIRT3信号通路发挥抗氧化和抗凋亡作用，从而改善公猪精子质量。我们的研究结果表明，NMN是一种新型的潜在公猪抗氧化饲料添加剂，可用于生产高质量的猪精液。
