Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
Cellulose and Paper Department, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, Egypt.
Colloids Surf B Biointerfaces. 2022 Jan;209(Pt 1):112172. doi: 10.1016/j.colsurfb.2021.112172. Epub 2021 Oct 20.
In this work, biocompatible, antimicrobial, and antiviral nanocomposites were prepared through two steps. In the first step, periodate oxidation of cellulose was performed to get dialdehyde cellulose (DAC). The second step included the reaction of DAC with sulfur-containing amino acids included Cysteine (Cys) and Methionine (Meth) in the presence of graphene oxide (GO). The prepared nanocomposites were characterized via FT-IR, SEM, TEM, and TGA. Antimicrobial and antiviral activities for all designed nanocomposites besides DAC were carried out. Both DAC/GO/Cys and DAC/GO/Meth exhibited a promising antimicrobial activity against Gram-negative (E. coli and P. aeruginosa), Gram-positive (B. subtilis and S. aureus), and unicellular fungi (C. Albicans and C. neoformans), while the DAC/GO/Cys/Meth nanocomposite was the lowest. Moreover, all designed nanocomposites have a strong antiviral activity against Herpes simplex virus 1(HSV-1) at minimum nontoxic concentration. Additionally, Computational procedures and Molecular docking showed the reactivity and stability of the molecules that have biological activity against Gram-positive, Gram-negative, and HSV-1. As well as DAC incorporation with amino acid enhanced their reactivity and their interaction.
在这项工作中，通过两步法制备了生物相容性、抗菌和抗病毒纳米复合材料。第一步是通过过碘酸盐氧化纤维素得到二醛纤维素（DAC）。第二步包括在氧化石墨烯（GO）存在下，DAC 与含硫氨基酸半胱氨酸（Cys）和蛋氨酸（Meth）反应。通过 FT-IR、SEM、TEM 和 TGA 对制备的纳米复合材料进行了表征。对所有设计的纳米复合材料（包括 DAC）进行了抗菌和抗病毒活性测试。DAC/GO/Cys 和 DAC/GO/Meth 对革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）、革兰氏阳性菌（枯草芽孢杆菌和金黄色葡萄球菌）和单细胞真菌（白色念珠菌和新型隐球菌）均表现出良好的抗菌活性，而 DAC/GO/Cys/Meth 纳米复合材料的抗菌活性最低。此外，所有设计的纳米复合材料在最低非毒性浓度下均对单纯疱疹病毒 1（HSV-1）具有很强的抗病毒活性。此外，计算程序和分子对接显示了对革兰氏阳性菌、革兰氏阴性菌和 HSV-1 具有生物活性的分子的反应性和稳定性。以及 DAC 与氨基酸的结合增强了它们的反应性和相互作用。
