揭示利用 Cladosporium cladosporioides CBS 174.62 生物合成的淀粉/银-硒纳米复合材料的生物活性。

Unveiling biological activities of biosynthesized starch/silver-selenium nanocomposite using Cladosporium cladosporioides CBS 174.62.

机构信息

Microbiology and Immunology Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, 11884, Egypt.

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt.

出版信息

BMC Microbiol. 2024 Mar 8;24(1):78. doi: 10.1186/s12866-024-03228-1.

DOI:10.1186/s12866-024-03228-1

PMID:38459502

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10921769/

Abstract

BACKGROUND AND OBJECTIVES

Microbial cells capability to tolerate the effect of various antimicrobial classes represent a major worldwide health concern. The flexible and multi-components nanocomposites have enhanced physicochemical characters with several improved properties. Thus, different biological activities of biosynthesized starch/silver-selenium nanocomposite (St/Ag-Se NC) were assessed.

METHODOLOGY

The St/Ag-Se NC was biosynthesized using Cladosporium cladosporioides CBS 174.62 (C. cladosporioides) strain. The shape and average particle size were investigated using scanning electron microscope (SEM) and high-resolution transmission electron microscope (HR-TEM), respectively. On the other hand, the St/Ag-Se NC effect on two cancer cell lines and red blood cells (RBCs) was evaluated and its hydrogen peroxide (HO) scavenging effect was assessed. Moreover, its effects on various microbial species in both planktonic and biofilm growth forms were examined.

RESULTS

The St/Ag-Se NC was successfully biosynthesized with oval and spherical shape and a mean particle diameter of 67.87 nm as confirmed by the HR-TEM analysis. St/Ag-Se NC showed promising anticancer activity toward human colorectal carcinoma (HCT-116) and human breast cancer (MCF-7) cell lines where IC were 21.37 and 19.98 µg/ml, respectively. Similarly, little effect on RBCs was observed with low nanocomposite concentration. As well, the highest nanocomposite HO scavenging activity (42.84%) was recorded at a concentration of 2 mg/ml. Additionally, Staphylococcus epidermidis (S. epidermidis) ATCC 12,228 and Candida albicans (C. albicans) ATCC 10,231 were the highly affected bacterial and fungal strains with minimum inhibitory concentrations (MICs) of 18.75 and 50 µg/ml, respectively. Moreover, the noticeable effect of St/Ag-Se NC on microbial biofilm was concentration dependent. A high biofilm suppression percentage, 87.5% and 68.05%, were recorded with S. epidermidis and Staphylococcus aureus (S. aureus) when exposed to 1 mg/ml and 0.5 mg/ml, respectively.

CONCLUSION

The biosynthesized St/Ag-Se NC showed excellent antioxidant activity, haemocompatibility, and anti-proliferative effect at low concentrations. Also, it exhibited promising antimicrobial and antibiofilm activities.

摘要

背景与目的

微生物细胞耐受各种抗菌类别的能力是一个全球性的健康问题。具有灵活和多组分的纳米复合材料具有增强的物理化学特性和多种改进的性能。因此，评估了生物合成淀粉/银-硒纳米复合材料（St/Ag-Se NC）的不同生物学活性。

方法

使用枝孢霉（C. cladosporioides）CBS 174.62 菌株（C. cladosporioides）生物合成 St/Ag-Se NC。使用扫描电子显微镜（SEM）和高分辨率透射电子显微镜（HR-TEM）分别研究了其形状和平均粒径。另一方面，评估了 St/Ag-Se NC 对两种癌细胞系和红细胞（RBC）的影响，并评估了其对过氧化氢（HO）的清除作用。此外，还研究了其对浮游和生物膜生长形式的各种微生物的影响。

结果

通过 HR-TEM 分析证实，St/Ag-Se NC 成功地以椭圆形和球形形状合成，平均粒径为 67.87nm。St/Ag-Se NC 对人结肠直肠癌细胞（HCT-116）和人乳腺癌细胞（MCF-7）表现出有希望的抗癌活性，其 IC 分别为 21.37 和 19.98μg/ml。同样，在低纳米复合材料浓度下对 RBC 的影响很小。同样，在 2mg/ml 浓度下，纳米复合材料 HO 清除活性（42.84%）最高。此外，表皮葡萄球菌（S. epidermidis）ATCC 12,228 和白色念珠菌（C. albicans）ATCC 10,231 是受影响最大的细菌和真菌菌株，其最小抑菌浓度（MIC）分别为 18.75 和 50μg/ml。此外，St/Ag-Se NC 对微生物生物膜的显著作用取决于浓度。当暴露于 1mg/ml 和 0.5mg/ml 时，表皮葡萄球菌和金黄色葡萄球菌（S. aureus）的生物膜抑制率分别为 87.5%和 68.05%。