The Collaboration Unit for Field Epidemiology of State Key Laboratory of Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-origin and Vector-borne Diseases, Nanchang Center for Disease Control and Prevention, Nanchang 330038, PR China.
School of Public Health, Nanchang University, Jiangxi Provincial Key Laboratory of Preventive Medicine, Nanchang University, Nanchang 330006, PR China.
Am J Trop Med Hyg. 2021 Oct 29;106(1):127-131. doi: 10.4269/ajtmh.21-0732.
This article aims to understand the changes in the detection rates of H5, H7, and H9 subtypes of avian influenza viruses (AIVs) in the live poultry markets (LPMs) in Nanchang City, Jiangxi Province, before and after the outbreak of the COVID-19. From 2019 to 2020, we monitored the LPM and collected specimens, using real-time reverse transcription polymerase chain reaction technology to detect the nucleic acid of type A AIV in the samples. The H5, H7, and H9 subtypes of influenza viruses were further classified for positive results. We analyzed 1,959 samples before and after the outbreak and found that the positive rates of avian influenza A virus (39.69%) and H9 subtype (30.66%) after the outbreak were significantly higher than before the outbreak (26.84% and 20.90%, respectively; P < 0.001). In various LPMs, the positive rate of H9 subtypes has increased significantly (P ≤ 0.001). Positive rates of the H9 subtype in duck, fecal, daub, and sewage samples, but not chicken samples, have increased to varying degrees. This study shows that additional measures are needed to strengthen the control of AIVs now that LPMs have reopened after the relaxing of COVID-19-related restrictions.
本文旨在了解 COVID-19 爆发前后江西省南昌市活禽市场（LPM）中 H5、H7 和 H9 亚型禽流感病毒（AIV）检测率的变化。我们在 2019 年至 2020 年期间监测了 LPM 并收集了样本，使用实时逆转录聚合酶链反应技术检测样本中 A 型 AIV 的核酸。对阳性结果进一步分类为流感病毒的 H5、H7 和 H9 亚型。我们分析了爆发前后的 1959 个样本，发现爆发后禽流感 A 病毒（39.69%）和 H9 亚型（30.66%）的阳性率明显高于爆发前（分别为 26.84%和 20.90%；P<0.001）。在各种 LPM 中，H9 亚型的阳性率显著增加（P≤0.001）。鸭、粪便、涂抹物和污水样本中 H9 亚型的阳性率有所增加，但鸡样本中 H9 亚型的阳性率没有增加。本研究表明，在 LPM 在 COVID-19 相关限制放宽后重新开放的情况下，需要采取额外措施加强对 AIV 的控制。
