Vicente Dos Santos Victor, Tixier Marie-Stephane
Montpellier SupAgro, Unité Mixte de Recherche Centre de Biologie pour la Gestion des Populations (INRA/IRD/CIRAD/Montpellier SupAgro), Campus International de Baillarguet, CS 30016, Montferrier-sur-Lez Cedex, 34988, France.
Cladistics. 2017 Jun;33(3):251-267. doi: 10.1111/cla.12166. Epub 2016 May 8.
The use of molecular markers for resolving systematics issues has improved our knowledge of life history. However, for the taxa studied herein-the predatory mite family Phytoseiidae-molecular phylogeny is impeded by a lack of suitable markers for deeper taxonomic levels. This study aims (i) to establish DNA amplification protocols for molecular markers known to resolve supraspecific nodes in other taxa, (ii) to determine their individual performance in assessing the clustering of species, genera, tribes and subfamilies, and (iii) to characterize the additional information provided when markers are concatenated. A new phylogenetic index is proposed based on ecological concepts, considering trees as a community of nodes. New and efficient protocols for DNA amplification of six molecular markers are provided. The concatenated tree globally provides more robust and reliable information, especially for deeper nodes. However, for assessing species identification and within-genera phylogenies, the combined use of six markers does not seem necessary, underlining the need to resize experiments depending on their taxonomic objectives. Finally, this study lays the methodological foundations with which to test the present Phytoseiidae classification as the first phylogeny obtained shows incongruence with the present morphological classification.
使用分子标记来解决系统发育问题，已经增进了我们对生活史的了解。然而，对于本文所研究的类群——捕食螨科（Phytoseiidae）——分子系统发育受到缺乏适用于更高级分类水平的标记的阻碍。本研究旨在：（i）为已知能解决其他类群超特定节点问题的分子标记建立DNA扩增方案；（ii）确定它们在评估物种、属、族和亚科聚类方面的各自表现；（iii）描述串联标记时所提供的额外信息。基于生态概念提出了一种新的系统发育指数，将树视为节点的群落。提供了六种分子标记的新的高效DNA扩增方案。串联树总体上提供了更稳健和可靠的信息，尤其是对于更深层次的节点。然而，对于评估物种鉴定和属内系统发育，六种标记的联合使用似乎并非必要，这凸显了根据分类目标调整实验规模的必要性。最后，本研究奠定了方法学基础，用以检验当前的捕食螨科分类，因为所获得的首个系统发育结果与当前的形态学分类不一致。
