Fang Xiaoduan, Li Jun, Mahmood Syed Usman, Fidelis Nwanade Chuks, Meng Jianglei
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China.
Insects. 2025 Sep 10;16(9):950. doi: 10.3390/insects16090950.
In a survey of Bajiaozhai National Forest Park (Guilin, China), several specimens of an sp. were collected. These specimens were very similar to and , based on morphological observations. However, some morphological characters, such as the body size, number of solenostomes on the dorsal plate, calyx shape of the spermatheca, the shape and number of metapodal platelet, teeth number on the fixed digit, length of setae j3, and macroseta Seg IV, Sti IV, and St IV were different between these specimens and and . To ascertain whether these morphological differences were interspecific or intraspecific variations, molecular analyses were conducted using mitochondrial DNA COI, 12S rRNA, and nuclear ITS markers. Based on the three molecular markers, minimal genetic distances were observed (COI 0-4%, 12S rRNA 0-2%, and ITS 0%) among the putative sp., (collected from in IZGAS and from and in Shaoguan City), and (collected from in IZGAS). was used as the outgroup. Using the maximum likelihood method, the phylogenetic tree showed that these specimens of sp., , and clustered in a single clade. Therefore, we propose that this putative sp. is , and is a junior synonym of . This study demonstrates the importance of integrative taxonomy for the proper identification of phytoseiid mites.
在对八角寨国家森林公园（中国桂林）的一项调查中，采集到了几种某螨种的标本。基于形态学观察，这些标本与[具体螨种1]和[具体螨种2]非常相似。然而，这些标本与[具体螨种1]和[具体螨种2]之间在一些形态特征上存在差异，如虫体大小、背板上的气门沟数量、受精囊的萼形状、后足板的形状和数量、固定趾上的齿数、刚毛j3的长度以及第四对足的巨毛、第四对足胫节刚毛和第四对足跗节刚毛。为了确定这些形态差异是种间差异还是种内变异，使用线粒体DNA COI、12S rRNA和核ITS标记进行了分子分析。基于这三个分子标记，在假定的某螨种、[具体螨种1]（从IZGAS的[具体地点1]采集以及从韶关市的[具体地点2]和[具体地点3]采集）和[具体螨种2]（从IZGAS的[具体地点4]采集）之间观察到最小遗传距离（COI为0 - 4%，12S rRNA为0 - 2%，ITS为0%）。[用作外群的螨种]被用作外群。使用最大似然法，系统发育树显示这些某螨种、[具体螨种1]和[具体螨种2]的标本聚在一个单系分支中。因此，我们提出这个假定的某螨种是[具体螨种2]，并且[具体螨种1]是[具体螨种2]的次异名。本研究证明了综合分类学在正确鉴定植绥螨中的重要性。
