Departamento de Ciencias Computacionales, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Guadalajara 44430, México.
Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C, Zapopan 45019, México.
G3 (Bethesda). 2022 Jan 4;12(1). doi: 10.1093/g3journal/jkab347.
The yeast Kluyveromyces marxianus SLP1 has the potential for application in biotechnological processes because it can metabolize several sugars and produce high-value metabolites. K. marxianus SLP1 is a thermotolerant yeast isolated from the mezcal process, and it is tolerant to several cell growth inhibitors such as saponins, furan aldehydes, weak acids, and phenolics compounds. The genomic differences between dairy and nondairy strains related to K. marxianus variability are a focus of research attention, particularly the pathways leading this species toward polyploidy. We report the diploid genome assembly of K. marxianus SLP1 nonlactide strain into 32 contigs to reach a size of ∼12 Mb (N50 = 1.3 Mb) and a ∼39% GC content. Genome size is consistent with the k-mer frequency results. Genome annotation by Funannotate estimated 5000 genes in haplotype A and 4910 in haplotype B. The enriched annotated genes by ontology show differences between alleles in biological processes and cellular component. The analysis of variants related to DMKU3 and between haplotypes shows changes in LAC12 and INU1, which we hypothesize can impact carbon source performance. This report presents the first polyploid K. marxianus strain recovered from nonlactic fermenting medium.
马克斯克鲁维酵母 SLP1 具有在生物技术过程中应用的潜力，因为它可以代谢多种糖并产生高价值的代谢物。马克斯克鲁维酵母 SLP1 是一种从梅斯卡尔工艺中分离出来的耐热酵母，它对几种细胞生长抑制剂（如皂素、呋喃醛、弱酸和酚类化合物）具有耐受性。与马克斯克鲁维酵母变异性相关的乳制品和非乳制品菌株之间的基因组差异是研究关注的焦点，特别是导致该物种向多倍体进化的途径。我们报告了非乳酰基 K. marxianus SLP1 非乳糖菌株的二倍体基因组组装成 32 个连续体，大小约为 12 Mb（N50=1.3 Mb），GC 含量约为 39%。基因组大小与 k-mer 频率结果一致。通过 Funannotate 进行基因组注释，在单倍型 A 中估计有 5000 个基因，在单倍型 B 中估计有 4910 个基因。通过本体论富集注释基因显示等位基因之间在生物过程和细胞组成方面存在差异。与 DMKU3 相关的变体和单倍型之间的分析表明 LAC12 和 INU1 发生了变化，我们假设这可能会影响碳源性能。本报告首次报道了从非乳发酵培养基中回收的多倍体马克斯克鲁维酵母菌株。
