College of Agronomy, Liaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252000, China.
J Insect Sci. 2021 Sep 1;21(5). doi: 10.1093/jisesa/ieab081.
A lipidomics approach based on liquid chromatography-tandem mass spectrometry (LC-MS) was applied to analyze the molecular-level mechanism of lipid deposition in Hermetia illucens (H. illucens) larvae fed food waste (FW) at different days of age. The H. illucens larvae reared on FW substrates generally became larger, heavier, and fatter at 5-15 d of age. A large amount of glycerolipids (GL) were deposited, while glycerophospholipids (GP), sphingolipids, and derivatized lipids became relatively less abundant during the growth stage of the larvae. Forty-three subclasses of 3,205 lipid molecules were identified in larvae, and 139 lipids (79 upregulated and 60 downregulated during larval growth and development) were identified as potential biomarkers (variable importance in projection > 1; P < 0.05). The differential lipids were mainly enriched in 19 metabolic pathways, of which 9 metabolic pathways related to lipids, including GL and GP metabolisms. The results demonstrate that the lipid composition and mechanisms changed during the growth and development stage of H. illucens larvae. To the best of our knowledge, this is the first work exploring the molecular-level mechanism of lipid deposition during the growth and development stage of H. illucens larvae. The findings provide novel information for determining and utilizing the nutritional value of H. illucens larvae.
基于液相色谱-串联质谱（LC-MS）的脂质组学方法被应用于分析在不同日龄时以食物垃圾（FW）为食的 Hermetia illucens（H. illucens）幼虫中脂质沉积的分子水平机制。在 FW 基质中饲养的 H. illucens 幼虫在 5-15 天龄时通常会变得更大、更重、更胖。大量甘油酯（GL）被沉积，而甘油磷脂（GP）、鞘脂和衍生脂质在幼虫生长阶段变得相对较少。在幼虫中鉴定出了 43 个亚类的 3205 种脂质分子，其中有 139 种脂质（在幼虫生长和发育过程中上调了 79 种，下调了 60 种）被鉴定为潜在的生物标志物（投影变量重要性>1；P<0.05）。差异脂质主要富集在 19 条代谢途径中，其中 9 条与脂质相关的代谢途径，包括 GL 和 GP 代谢。结果表明，在 H. illucens 幼虫的生长和发育阶段，脂质组成和机制发生了变化。据我们所知，这是首次探索 H. illucens 幼虫生长和发育阶段脂质沉积的分子水平机制的工作。这些发现为确定和利用 H. illucens 幼虫的营养价值提供了新的信息。
