Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Data Science Research Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Nucleic Acids Res. 2021 Nov 18;49(20):11447-11458. doi: 10.1093/nar/gkab956.
DNA-protein interactions play essential roles in all living cells. Understanding of how features embedded in the DNA sequence affect specific interactions with proteins is both challenging and important, since it may contribute to finding the means to regulate metabolic pathways involving DNA-protein interactions. Using a massive experimental benchmark dataset of binding scores for DNA sequences and a machine learning workflow, we describe the binding to DNA of T7 primase, as a model system for specific DNA-protein interactions. Effective binding of T7 primase to its specific DNA recognition sequences triggers the formation of RNA primers that serve as Okazaki fragment start sites during DNA replication.
DNA-蛋白质相互作用在所有活细胞中都起着至关重要的作用。了解 DNA 序列中嵌入的特征如何影响与蛋白质的特定相互作用，既具有挑战性，又很重要，因为这可能有助于找到调节涉及 DNA-蛋白质相互作用的代谢途径的方法。我们使用大量的 DNA 序列结合评分实验基准数据集和机器学习工作流程，描述了 T7 引发酶与 DNA 的结合情况，T7 引发酶是特定 DNA-蛋白质相互作用的模型系统。T7 引发酶与特定 DNA 识别序列的有效结合触发了 RNA 引物的形成，这些引物在 DNA 复制过程中充当冈崎片段起始位点。
