Aiewviriyasakul Katesuda, Bunterngsook Benjarat, Lekakarn Hataikarn, Sritusnee Wipawee, Kanokratana Pattanop, Champreda Verawat
Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Road, Khlong Luang, Pathumthani, 12120, Thailand.
Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Campus, Phahonyothin Road, Khlong Luang, Pathumthani, 12120, Thailand.
Biotechnol Lett. 2021 Dec;43(12):2299-2310. doi: 10.1007/s10529-021-03202-1. Epub 2021 Oct 31.
To develop an endo-β-1,4-xylanase with high specificity for production of prebiotic xylooligosaccharides that optimally works at moderate temperature desirable to reduce the energy cost in the production process.
The xylB gene, encoding for a glycosyl hydrolase family 11 xylanase from a thermoresistant fungus, Aspergillus niger BCC14405 was expressed in a methylotrophic yeast P. pastoris KM71 in a secreted form. The recombinant XylB showed a high specific activity of 3852 and 169 U mg protein on beechwood xylan and arabinoxylan, respectively with no detectable side activities against different forms of cellulose (Avicel Ò PH101 microcrystalline cellulose, phosphoric acid swollen cellulose and carboxymethylcellulose). The enzyme worked optimally at 45 °C, pH 6.0. It showed a specific cleavage pattern by releasing xylobiose (X2) as the major product from xylooligosaccharides (X3 to X6) substrates. The highest XOS yield of 708 mg g substrate comprising X2, X3 and X6 was obtained from beechwood xylan hydrolysis.
The enzyme is potent for XOS production and for saccharification of lignocellulosic biomass.
开发一种具有高特异性的内切-β-1,4-木聚糖酶，用于生产益生元木寡糖，该酶在适宜的中等温度下发挥最佳作用，以降低生产过程中的能源成本。
编码来自耐热真菌黑曲霉BCC14405的糖基水解酶家族11木聚糖酶的xylB基因，以分泌形式在甲基营养酵母巴斯德毕赤酵母KM71中表达。重组木聚糖酶XylB对山毛榉木聚糖和阿拉伯木聚糖分别表现出3852和169 U mg蛋白的高比活性，对不同形式的纤维素（微晶纤维素Avicel® PH101、磷酸膨胀纤维素和羧甲基纤维素）没有可检测到的副活性。该酶在45°C、pH 6.0时发挥最佳作用。它通过从木寡糖（X3至X6）底物中释放木二糖（X2）作为主要产物，表现出特定的切割模式。从山毛榉木聚糖水解中获得了最高708 mg g底物的木寡糖产量，包括X2、X3和X6。
该酶在生产木寡糖和木质纤维素生物质糖化方面具有潜力。
