Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.
Environ Sci Pollut Res Int. 2022 Jan;29(3):3171-3183. doi: 10.1007/s11356-021-17164-4. Epub 2021 Oct 31.
Soil microbiome is a dynamic micro-ecosystem driving and fine-tuning several biological processes in the global macro-ecosystems. Its tremendous potential towards mediating sustainability in the ecosystem necessitates the urgent need to store it optimally and efficiently as "next-generation biologicals" for future applications via soil transplantation. The challenge, therefore, is to devise a strategy for the storage of soil microbiome such that its "functionality" is preserved for later application. This review discusses the current endeavours made towards storage of the soil microbiome. The methods for assessing the integrity of soil microbiome by targeting the structural diversity and functional potential of the preserved microbiomes have also been discussed. Further, the success stories related to the storage of fecal microbiome for application in transplants have also been highlighted. This is done primarily with the objective of learning lessons, and parallel application of the knowledge gained, in bringing about improvement in the research domain of soil microbiome storage. Subsequently, the limitations of current techniques of preservation have also been delineated. Further, the open questions in the area have been critically discussed. In conclusion, possible alternatives for storage, comprehensive analyses of the composition of the stored microbiome and their potential have been presented.
土壤微生物组是一个动态的微型生态系统，它在全球宏观生态系统中驱动和微调着几个生物学过程。它在生态系统中具有巨大的可持续发展潜力，因此迫切需要通过土壤移植，将其作为“下一代生物制剂”进行最优和高效的储存，以便未来应用。因此，挑战在于设计一种储存土壤微生物组的策略，以保持其“功能”，以备将来应用。本综述讨论了目前在储存土壤微生物组方面所做的努力。还讨论了通过靶向保存的微生物组的结构多样性和功能潜力来评估土壤微生物组完整性的方法。此外，还强调了与粪便微生物组储存相关的成功案例，以便在移植中应用。这样做的主要目的是吸取经验教训，并平行应用所获得的知识，以提高土壤微生物组储存研究领域的水平。随后，还描述了当前保存技术的局限性。此外，还对该领域的开放性问题进行了批判性讨论。总之，提出了可能的替代储存方法、储存微生物组的组成及其潜在应用的综合分析。
