• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

不同的青枯雷尔氏菌效应子 RipAW 表位被两种烟草识别，并引发免疫反应。

Different epitopes of Ralstonia solanacearum effector RipAW are recognized by two Nicotiana species and trigger immune responses.

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.

Shaanxi Key State Laboratory of Crop Heterosis, Northwest A&F University, Yangling, China.

出版信息

Mol Plant Pathol. 2022 Feb;23(2):188-203. doi: 10.1111/mpp.13153. Epub 2021 Oct 31.

DOI:10.1111/mpp.13153
PMID:34719088
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8743020/
Abstract

Diverse pathogen effectors convergently target conserved components in plant immunity guarded by intracellular nucleotide-binding domain leucine-rich repeat receptors (NLRs) and activate effector-triggered immunity (ETI), often causing cell death. Little is known of the differences underlying ETI in different plants triggered by the same effector. In this study, we demonstrated that effector RipAW triggers ETI on Nicotiana benthamiana and Nicotiana tabacum. Both the first 107 amino acids (N ) and RipAW E3-ligase activity are required but not sufficient for triggering ETI on N. benthamiana. However, on N. tabacum, the N fragment is essential and sufficient for inducing cell death. The first 60 amino acids of the protein are not essential for RipAW-triggered cell death on either N. benthamiana or N. tabacum. Furthermore, simultaneous mutation of both R75 and R78 disrupts RipAW-triggered ETI on N. tabacum, but not on N. benthamiana. In addition, N. tabacum recognizes more RipAW orthologs than N. benthamiana. These data showcase the commonalities and specificities of RipAW-activated ETI in two evolutionally related species, suggesting Nicotiana species have acquired different abilities to perceive RipAW and activate plant defences during plant-pathogen co-evolution.

摘要

不同的病原体效应子通过细胞内核苷酸结合域富含亮氨酸重复受体（NLRs）靶向植物免疫中保守的成分，并激活效应子触发的免疫（ETI），通常导致细胞死亡。对于由相同效应子触发的不同植物中的 ETI 差异，人们知之甚少。在这项研究中，我们证明了效应子 RipAW 在 Nicotiana benthamiana 和 Nicotiana tabacum 上触发 ETI。前 107 个氨基酸（N 端）和 RipAW E3 连接酶活性对于在 N. benthamiana 上触发 ETI 是必需的，但不是充分的。然而，在 N. tabacum 上，N 片段对于诱导细胞死亡是必需的和充分的。该蛋白的前 60 个氨基酸对于在 N. benthamiana 或 N. tabacum 上的 RipAW 触发细胞死亡不是必需的。此外，同时突变 R75 和 R78 会破坏 N. tabacum 上 RipAW 触发的 ETI，但不会破坏 N. benthamiana 上的 ETI。此外，N. tabacum 识别的 RipAW 同源物比 N. benthamiana 多。这些数据展示了两种进化上相关的物种中 RipAW 激活的 ETI 的共性和特异性，表明 Nicotiana 物种在植物-病原体共同进化过程中获得了不同的感知 RipAW 和激活植物防御的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/80219d69d8aa/MPP-23-188-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/2e73576247ae/MPP-23-188-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/878398973daf/MPP-23-188-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/3ffdef63f516/MPP-23-188-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/735e8805b96c/MPP-23-188-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/a59e98cbf6c5/MPP-23-188-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/80219d69d8aa/MPP-23-188-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/2e73576247ae/MPP-23-188-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/878398973daf/MPP-23-188-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/3ffdef63f516/MPP-23-188-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/735e8805b96c/MPP-23-188-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/a59e98cbf6c5/MPP-23-188-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4d1/8743020/80219d69d8aa/MPP-23-188-g004.jpg

相似文献

1
Different epitopes of Ralstonia solanacearum effector RipAW are recognized by two Nicotiana species and trigger immune responses.不同的青枯雷尔氏菌效应子 RipAW 表位被两种烟草识别，并引发免疫反应。
Mol Plant Pathol. 2022 Feb;23(2):188-203. doi: 10.1111/mpp.13153. Epub 2021 Oct 31.
2
Ubiquitin E3 ligase activity of effector RipAW is not essential for induction of plant defense in .效应蛋白RipAW的泛素E3连接酶活性对于诱导植物防御并非必需。
Front Microbiol. 2023 May 24;14:1201444. doi: 10.3389/fmicb.2023.1201444. eCollection 2023.
3
Ralstonia solanacearum novel E3 ubiquitin ligase (NEL) effectors RipAW and RipAR suppress pattern-triggered immunity in plants.青枯雷尔氏菌新型E3泛素连接酶（NEL）效应蛋白RipAW和RipAR抑制植物中的模式触发免疫。
Microbiology (Reading). 2017 Jul;163(7):992-1002. doi: 10.1099/mic.0.000495. Epub 2017 Jul 21.
4
The type III effector RipB from Ralstonia solanacearum RS1000 acts as a major avirulence factor in Nicotiana benthamiana and other Nicotiana species.青枯雷尔氏菌RS1000的III型效应蛋白RipB在本氏烟草和其他烟草属物种中作为主要无毒因子发挥作用。
Mol Plant Pathol. 2019 Sep;20(9):1237-1251. doi: 10.1111/mpp.12824. Epub 2019 Jun 20.
5
The Type III Effector RipAW Targets the Immune Receptor Complex to Suppress PAMP-Triggered Immunity.III 型效应器 RipAW 靶向免疫受体复合物以抑制 PAMP 触发的免疫。
Int J Mol Sci. 2023 Dec 22;25(1):183. doi: 10.3390/ijms25010183.
6
Ralstonia solanacearum Type III Effector RipAC Targets SGT1 to Suppress Effector-Triggered Immunity.青枯雷尔氏菌Ⅲ型效应物 RipAC 靶向 SGT1 抑制效应子触发的免疫。
Plant Cell Physiol. 2021 Feb 4;61(12):2067-2076. doi: 10.1093/pcp/pcaa122.
7
A plasma membrane nucleotide-binding leucine-rich repeat receptor mediates the recognition of the Ralstonia pseudosolanacearum effector RipY in Nicotiana benthamiana.质膜核苷酸结合富含亮氨酸重复受体介导拟南芥对丁香假单胞菌效应因子 RipY 的识别。
Plant Commun. 2023 Nov 13;4(6):100640. doi: 10.1016/j.xplc.2023.100640. Epub 2023 Jun 21.
8
Ralstonia solanacearum type III effector RipV2 encoding a novel E3 ubiquitin ligase (NEL) is required for full virulence by suppressing plant PAMP-triggered immunity.青枯雷尔氏菌 III 型效应因子 RipV2 编码一种新型 E3 泛素连接酶（NEL），通过抑制植物的 PAMP 触发免疫来实现完全毒力。
Biochem Biophys Res Commun. 2021 Apr 23;550:120-126. doi: 10.1016/j.bbrc.2021.02.082. Epub 2021 Mar 7.
9
Intra-strain Elicitation and Suppression of Plant Immunity by Type-III Effectors in .III 型效应因子在 中诱导和抑制植物免疫的同种菌株激发和抑制作用。
Plant Commun. 2020 Jan 21;1(4):100025. doi: 10.1016/j.xplc.2020.100025. eCollection 2020 Jul 13.
10
Effector XopQ-induced stromule formation in Nicotiana benthamiana depends on ETI signaling components ADR1 and NRG1.效应因子 XopQ 诱导 Nicotiana benthamiana 中 stromule 的形成依赖于 ET 信号成分 ADR1 和 NRG1。
Plant Physiol. 2023 Jan 2;191(1):161-176. doi: 10.1093/plphys/kiac481.

引用本文的文献

1
Bacteriostatic Activity and Resistance Mechanism of Extract Against in Pepper.辣椒提取物对[具体对象未给出]的抑菌活性及抗性机制
Plants (Basel). 2025 Feb 20;14(5):651. doi: 10.3390/plants14050651.
2
Antibacterial Activity and Mechanism of Three Root Exudates from Mulberry Seedlings against .三种桑树苗根分泌物对……的抗菌活性及作用机制
Plants (Basel). 2024 Feb 8;13(4):482. doi: 10.3390/plants13040482.
3
The Type III Effector RipAW Targets the Immune Receptor Complex to Suppress PAMP-Triggered Immunity.III 型效应器 RipAW 靶向免疫受体复合物以抑制 PAMP 触发的免疫。

本文引用的文献

1
A bacterial effector protein uncovers a plant metabolic pathway involved in tolerance to bacterial wilt disease.一种细菌效应蛋白揭示了植物代谢途径参与耐受细菌性萎蔫病。
Mol Plant. 2021 Aug 2;14(8):1281-1296. doi: 10.1016/j.molp.2021.04.014. Epub 2021 May 1.
2
Ralstonia solanacearum type III effector RipV2 encoding a novel E3 ubiquitin ligase (NEL) is required for full virulence by suppressing plant PAMP-triggered immunity.青枯雷尔氏菌 III 型效应因子 RipV2 编码一种新型 E3 泛素连接酶（NEL），通过抑制植物的 PAMP 触发免疫来实现完全毒力。
Biochem Biophys Res Commun. 2021 Apr 23;550:120-126. doi: 10.1016/j.bbrc.2021.02.082. Epub 2021 Mar 7.
3
Int J Mol Sci. 2023 Dec 22;25(1):183. doi: 10.3390/ijms25010183.
4
Ubiquitin E3 ligase activity of effector RipAW is not essential for induction of plant defense in .效应蛋白RipAW的泛素E3连接酶活性对于诱导植物防御并非必需。
Front Microbiol. 2023 May 24;14:1201444. doi: 10.3389/fmicb.2023.1201444. eCollection 2023.
5
Three amino acid residues are required for the recognition of RipTPS in .识别RipTPS需要三个氨基酸残基。
Front Plant Sci. 2022 Oct 13;13:1040826. doi: 10.3389/fpls.2022.1040826. eCollection 2022.
6
The NEL Family of Bacterial E3 Ubiquitin Ligases.细菌 E3 泛素连接酶 NEL 家族。
Int J Mol Sci. 2022 Jul 13;23(14):7725. doi: 10.3390/ijms23147725.
Intra-strain Elicitation and Suppression of Plant Immunity by Type-III Effectors in .
III 型效应因子在 中诱导和抑制植物免疫的同种菌株激发和抑制作用。
Plant Commun. 2020 Jan 21;1(4):100025. doi: 10.1016/j.xplc.2020.100025. eCollection 2020 Jul 13.
4
A bacterial effector protein prevents MAPK-mediated phosphorylation of SGT1 to suppress plant immunity.一种细菌效应蛋白通过阻止 MAPK 介导的 SGT1 磷酸化来抑制植物免疫。
PLoS Pathog. 2020 Sep 25;16(9):e1008933. doi: 10.1371/journal.ppat.1008933. eCollection 2020 Sep.
5
The large, diverse, and robust arsenal of Ralstonia solanacearum type III effectors and their in planta functions.根癌农杆菌大型、多样且强大的 III 型效应子库及其在植物体内的功能。
Mol Plant Pathol. 2020 Oct;21(10):1377-1388. doi: 10.1111/mpp.12977. Epub 2020 Aug 8.
6
A Bacterial Effector Protein Hijacks Plant Metabolism to Support Pathogen Nutrition.一种细菌效应蛋白劫持植物代谢以支持病原体营养。
Cell Host Microbe. 2020 Oct 7;28(4):548-557.e7. doi: 10.1016/j.chom.2020.07.003. Epub 2020 Jul 30.
7
Plant Immunity: Danger Perception and Signaling.植物免疫：危险感知与信号转导。
Cell. 2020 May 28;181(5):978-989. doi: 10.1016/j.cell.2020.04.028. Epub 2020 May 21.
8
Stories of Salicylic Acid: A Plant Defense Hormone.水杨酸的故事：一种植物防御激素。
Trends Plant Sci. 2020 Jun;25(6):549-565. doi: 10.1016/j.tplants.2020.01.004. Epub 2020 Feb 12.
9
The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana.雷尔氏菌效应子 RipI 通过与本氏烟中的 bHLH93 转录因子相互作用诱导防御反应。
Mol Plant Pathol. 2020 Jul;21(7):999-1004. doi: 10.1111/mpp.12937. Epub 2020 Apr 13.
10
Four bottlenecks restrict colonization and invasion by the pathogen Ralstonia solanacearum in resistant tomato.四个瓶颈限制了病原菌青枯雷尔氏菌在抗性番茄中的定殖和侵染。
J Exp Bot. 2020 Mar 25;71(6):2157-2171. doi: 10.1093/jxb/erz562.