Escola de Veterinária e Zootecnia, Universidade Federal de Goiás - UFG, Goiânia, Goiás, Brazil.
Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, Minas Gerais, Brazil.
Vet Clin Pathol. 2021 Dec;50(4):495-500. doi: 10.1111/vcp.13019. Epub 2021 Oct 31.
A point-of-care device that can provide immediate and reliable hemoglobin (Hb) concentrations and packed cell volumes (PCVs) would be useful in veterinary medicine.
We aimed to compare the use of a human device (Mission Plus; MP) with a gold standard (GS) method for measuring Hb concentrations and PCVs in cattle blood.
Blood samples from clinically healthy cattle (n = 122) were collected with or without an anticoagulant (K EDTA). The GS and MP methods were compared with correlation coefficients. Passing-Bablok regression analyses were also performed, and the acceptability judgment was completed using Bland-Altman plots.
The CV for Hb values obtained using the GS method, the MP device without K EDTA, and the MP device with K EDTA were approximately 2.70%, 1.70%, and 2.0%, respectively, whereas the CV for PCVs was 0.90%, 1.83%, and 2.05%, respectively. A positive correlation (97.5% confidence interval) was observed between the Hb concentrations and PCV values detected using the MP and GS techniques in blood with and without K EDTA. Bland-Altman plots showed agreement between the MP and GS methods. For Hb using blood collected with or without the addition of K EDTA, the mean differences were -0.87 g/dL (95% CI: 1.35; -3.96) and 0.08 g/dL (95% CI: 2.16, -1.99), respectively. For PCVs using blood collected with or without the addition of K EDTA, the mean differences were -3.75% (95% CI: 0.61. -8.12) and -0.88% (95% CI: 2.86, -4.62), respectively.
The MP device can be used to analyze Hb concentrations and PCVs in bovine blood to assist in field diagnoses.
一种能够即时、可靠地提供血红蛋白（Hb）浓度和红细胞压积（PCV）的即时检测设备，在兽医领域将具有重要应用价值。
本研究旨在比较使用人用设备（Mission Plus；MP）与金标准（GS）方法测量牛血中 Hb 浓度和 PCV 的效果。
采集 122 份来自临床健康牛的血液样本，分为抗凝和非抗凝两组。使用相关系数对 GS 方法和 MP 方法进行比较，同时进行 Passing-Bablok 回归分析，并使用 Bland-Altman 图进行可接受性判断。
使用 GS 方法、无 K EDTA 的 MP 设备和有 K EDTA 的 MP 设备获得的 Hb 值的 CV 分别约为 2.70%、1.70%和 2.0%，而 PCV 的 CV 分别为 0.90%、1.83%和 2.05%。无论血液是否添加 K EDTA，使用 MP 和 GS 技术检测 Hb 浓度和 PCV 值均呈现正相关（97.5%置信区间）。Bland-Altman 图显示 MP 方法与 GS 方法具有一致性。对于添加和不添加 K EDTA 的血液样本，Hb 的平均差值分别为-0.87 g/dL（95%CI：1.35；-3.96）和 0.08 g/dL（95%CI：2.16，-1.99）。对于添加和不添加 K EDTA 的血液样本，PCV 的平均差值分别为-3.75%（95%CI：0.61. -8.12）和-0.88%（95%CI：2.86，-4.62）。
MP 设备可用于分析牛血中的 Hb 浓度和 PCV，有助于现场诊断。
